Rat TIMP-2 ELISA Kit PicoKine®

Rat TIMP-2 ELISA Kit PicoKine® (96 Tests) . Quantitate Rat Timp2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

TIMP-2 gen is encoded by 5 exons spanning 83 kb of genomic DNA. TIMP-2 is 83 kilobase pairs (kb) long with exon-intron splicing sites located in preserved positions among the three members of the TIMP family. The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition. The standard product used in this kit is recombinant human TIMP-2 with the molecular mass of 22Kda and 194 amino acid.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 240 251 238 213 235 13.9 5.9% Sample 2 1342 1286 1409 1375 1353 45.35 3.3% Sample 3 4533 4407 4200 4479 4404 126.38 2.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 240 251 238 213 235 13.9 5.9% Sample 1 240 251 238 213 235 13.9 5.9% Sample 2 1342 1286 1409 1375 1353 45.35 3.3% Sample 2 1342 1286 1409 1375 1353 45.35 3.3% Sample 3 4533 4407 4200 4479 4404 126.38 2.8% Sample 3 4533 4407 4200 4479 4404 126.38 2.8%

Synonyms

Metalloproteinase inhibitor 2; Tissue inhibitor of metalloproteinases 2; TIMP-2; Timp2; Timp-2

Gene Name

TIMP2

Gene ID

29543

UniProt

P30121

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: C27-P220

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Angiogenesis, Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Protease Inhibitors, Proteolysis/Ubiquitin, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 240 1342 4533 227 1463 4769 Standard deviation 10.56 68.44 339.97 10.21 95.09 443.51 CV (%) 4.4% 9.3% 7.5% 4.5% 6.5% 9.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 240 1342 4533 227 1463 4769 Standard deviation 10.56 68.44 339.97 10.21 95.09 443.51 CV (%) 4.4% 9.3% 7.5% 4.5% 6.5% 9.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 240 1342 4533 227 1463 4769 Mean (pg/ml) 240 1342 4533 227 1463 4769 Standard deviation 10.56 68.44 339.97 10.21 95.09 443.51 Standard deviation 10.56 68.44 339.97 10.21 95.09 443.51 CV (%) 4.4% 9.3% 7.5% 4.5% 6.5% 9.3% CV (%) 4.4% 9.3% 7.5% 4.5% 6.5% 9.3%

Sensitivity

<10 pg/mL

Function

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

24356 MW

Specificity

Natural and recombinant rat TIMP-2

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Metalloproteinase inhibitor 2

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the protease inhibitor I35 (TIMP) family.

Protein Name

Metalloproteinase inhibitor 2

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.024 0.141 0.248 0.431 0.857 1.39 1.932 2.243 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.024 0.141 0.248 0.431 0.857 1.39 1.932 2.243 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.024 0.141 0.248 0.431 0.857 1.39 1.932 2.243 O.D. 0.024 0.141 0.248 0.431 0.857 1.39 1.932 2.243

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.