Rat HGF/Hepatocyte growth factor ELISA Kit PicoKine®

Hepatocyte growth factor (HGF) is the most potent mitogen for mature parenchymal hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. HGF has a relative molecular mass (Mr) of 82,000 and is a heterodimer composed of a large alpha-subunit of Mr 69,000 and a small beta-subunit of Mr 34,000.1 The protein consists of 728 amino acid residues, including a possible signal peptide at the N-terminus.2 HGF may serve as a paracrine mediator to control placental development and growth.3 This growth factor may play an important role as a paracrine mediator of the proliferation of melanocytes and endothelial cells, as well as cells of epithelial origin.4 The gene encoding the human HGF is assigned to human chromosome 7.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 223 205 208 224 215 8.57 3.9% Sample 2 1031 1143 985 1076 1058 58.31 5.5% Sample 3 4762 4701 5150 5432 5011 297.76 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 223 205 208 224 215 8.57 3.9% Sample 1 223 205 208 224 215 8.57 3.9% Sample 2 1031 1143 985 1076 1058 58.31 5.5% Sample 2 1031 1143 985 1076 1058 58.31 5.5% Sample 3 4762 4701 5150 5432 5011 297.76 5.9% Sample 3 4762 4701 5150 5432 5011 297.76 5.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 223 1031 4762 201 1018 4682 Standard deviation 12.48 78.35 371.43 12.46 90.6 412.01 CV (%) 7.6% 7.6% 7.8% 6.2% 8.9% 8.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 223 1031 4762 201 1018 4682 Standard deviation 12.48 78.35 371.43 12.46 90.6 412.01 CV (%) 7.6% 7.6% 7.8% 6.2% 8.9% 8.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 223 1031 4762 201 1018 4682 Mean (pg/ml) 223 1031 4762 201 1018 4682 Standard deviation 12.48 78.35 371.43 12.46 90.6 412.01 Standard deviation 12.48 78.35 371.43 12.46 90.6 412.01 CV (%) 7.6% 7.6% 7.8% 6.2% 8.9% 8.8% CV (%) 7.6% 7.6% 7.8% 6.2% 8.9% 8.8%

Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.028 0.097 0.189 0.35 0.685 1.166 1.727 2.029 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.028 0.097 0.189 0.35 0.685 1.166 1.727 2.029 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.028 0.097 0.189 0.35 0.685 1.166 1.727 2.029 O.D. 0.028 0.097 0.189 0.35 0.685 1.166 1.727 2.029

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.