Rat IGFBP5 ELISA Kit PicoKine®

Rat IGFBP5 ELISA Kit PicoKine® (96 Tests) . Quantitate Rat Igfbp5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Insulin-like growth factor-binding protein 5 is a protein that in humans is encoded by the IGFBP5 gene. The IGFBP5 gene was mapped to chromosome 2q33-q34. The expression of IGFBP5 by stable transfection and adenovirus-mediated infection was inhibitory to growth in 2 human breast cancer cell lines. Stable expression of IGFBP5 in the breast cancer cell lines also inhibited the formation and growth of tumors following injection in athymic mice. IGFBP5 is believed a growth inhibitor and proapoptotic agent in breast cancer cells.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 315 305 301 291 303 8.6 2.8% Sample 2 1184 1112 1004 1119 1104 64.58 5.8% Sample 3 5793 5090 4974 4943 5200 346.72 6.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 315 305 301 291 303 8.6 2.8% Sample 1 315 305 301 291 303 8.6 2.8% Sample 2 1184 1112 1004 1119 1104 64.58 5.8% Sample 2 1184 1112 1004 1119 1104 64.58 5.8% Sample 3 5793 5090 4974 4943 5200 346.72 6.6% Sample 3 5793 5090 4974 4943 5200 346.72 6.6%

Synonyms

Insulin-like growth factor-binding protein 5; IBP-5; IGF-binding protein 5; IGFBP-5; Igfbp5; Igfbp-5

Gene Name

IGFBP5

Gene ID

25285

UniProt

P24594

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L20-E271

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Mostly in kidney.

Applications

ELISA

Field of Research

Growth Factors/Hormones, Lipid Signaling, Signal Transduction, Signaling Pathway

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 315 1184 5793 328 1134 5772 Standard deviation 24.25 73.4 341.78 26.24 85.05 369.4 CV (%) 7.7% 6.2% 5.9% 8% 7.5% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 315 1184 5793 328 1134 5772 Standard deviation 24.25 73.4 341.78 26.24 85.05 369.4 CV (%) 7.7% 6.2% 5.9% 8% 7.5% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 315 1184 5793 328 1134 5772 Mean (pg/ml) 315 1184 5793 328 1134 5772 Standard deviation 24.25 73.4 341.78 26.24 85.05 369.4 Standard deviation 24.25 73.4 341.78 26.24 85.05 369.4 CV (%) 7.7% 6.2% 5.9% 8% 7.5% 6.4% CV (%) 7.7% 6.2% 5.9% 8% 7.5% 6.4%

Sensitivity

<10 pg/mL

Function

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

30298 MW

Specificity

Natural and recombinant rat IGFBP5

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Insulin-like growth factor-binding protein 5

Subcellular Location

Secreted.

Sequence Similarities

Contains 1 IGFBP N-terminal domain.

Protein Name

Insulin-like growth factor-binding protein 5

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.042 0.065 0.099 0.145 0.263 0.522 1.135 1.906 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.042 0.065 0.099 0.145 0.263 0.522 1.135 1.906 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.042 0.065 0.099 0.145 0.263 0.522 1.135 1.906 O.D. 0.042 0.065 0.099 0.145 0.263 0.522 1.135 1.906

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.