Rat Angiopoietin-1 ELISA Kit PicoKine®

Angiopoietin 1, also called ANG1 is a type of angiopoietin and is encoded by the gene ANGPT1. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. This gene was mapped to 8q23.1. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 311 293 312 282 299 12.61 4.2% Sample 2 1285 1071 1253 1292 1225 90.26 7.3% Sample 3 4930 5240 5287 4371 4957 365.07 7.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 311 293 312 282 299 12.61 4.2% Sample 1 311 293 312 282 299 12.61 4.2% Sample 2 1285 1071 1253 1292 1225 90.26 7.3% Sample 2 1285 1071 1253 1292 1225 90.26 7.3% Sample 3 4930 5240 5287 4371 4957 365.07 7.3% Sample 3 4930 5240 5287 4371 4957 365.07 7.3%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 311 1285 4930 287 1289 5041 Standard deviation 18.97 60.39 295.8 18.65 70.89 317.58 CV (%) 6.1% 4.7% 6% 6.5% 5.5% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 311 1285 4930 287 1289 5041 Standard deviation 18.97 60.39 295.8 18.65 70.89 317.58 CV (%) 6.1% 4.7% 6% 6.5% 5.5% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 311 1285 4930 287 1289 5041 Mean (pg/ml) 311 1285 4930 287 1289 5041 Standard deviation 18.97 60.39 295.8 18.65 70.89 317.58 Standard deviation 18.97 60.39 295.8 18.65 70.89 317.58 CV (%) 6.1% 4.7% 6% 6.5% 5.5% 6.3% CV (%) 6.1% 4.7% 6% 6.5% 5.5% 6.3%

Binds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.04 0.109 0.198 0.311 0.685 1.131 1.69 2.289 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.04 0.109 0.198 0.311 0.685 1.131 1.69 2.289 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.04 0.109 0.198 0.311 0.685 1.131 1.69 2.289 O.D. 0.04 0.109 0.198 0.311 0.685 1.131 1.69 2.289

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.