Rat RANK ELISA Kit PicoKine®

Receptor Activator of Nuclear Factor κ B (RANK), also known as TRANCE Receptor, is a type I membrane protein that is expressed on the surface of osteoclasts and is involved in their activation upon ligand binding. RANK is a recently described TNF receptor family member, and its ligand, RANKL, promote survival of dendritic cells and differentiation of osteoclasts. RANK contains 383 amino acids in its intracellular domain (residues 234-616), which contain three putative TRAF-binding domains (termed I, II, and III) . RANK interacts with various TRAFs through distinct motifs and activates NF-kappaB via a novel TRAF6 interaction motif, which then activates NIK, thus leading to NF-kappaB activation, whereas RANK most likely activates JNK through a TRAF2-interacting region in RANK. The standard in this kit is recombinant human RANK with the sequence of Q29-G213 aa. It is a dipolymer which compose of two chains, and the molecular weight of each is 48kda.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 209 194 185 217 201 12.49 6.2% Sample 2 514 489 548 539 522 23 4.4% Sample 3 1401 1406 1464 1575 1461 70.05 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 209 194 185 217 201 12.49 6.2% Sample 1 209 194 185 217 201 12.49 6.2% Sample 2 514 489 548 539 522 23 4.4% Sample 2 514 489 548 539 522 23 4.4% Sample 3 1401 1406 1464 1575 1461 70.05 4.7% Sample 3 1401 1406 1464 1575 1461 70.05 4.7%

Adapters, Atherosclerosis, Cardiovascular, Cytokines, Cytoskeleton/ECM, Extracellular Matrix, Growth Factors/Hormones, Immunology, Innate Immunity, Signal Transduction, Structures, TNF Superfamily, Transmembrane, Vascular Inflammation

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 209 514 1401 226 542 1518 Standard deviation 13.58 34.95 88.16 18.3 42.81 97.15 CV (%) 6.5% 6.8% 7.9% 8.1% 7.9% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 209 514 1401 226 542 1518 Standard deviation 13.58 34.95 88.16 18.3 42.81 97.15 CV (%) 6.5% 6.8% 7.9% 8.1% 7.9% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 209 514 1401 226 542 1518 Mean (pg/ml) 209 514 1401 226 542 1518 Standard deviation 13.58 34.95 88.16 18.3 42.81 97.15 Standard deviation 13.58 34.95 88.16 18.3 42.81 97.15 CV (%) 6.5% 6.8% 7.9% 8.1% 7.9% 6.4% CV (%) 6.5% 6.8% 7.9% 8.1% 7.9% 6.4%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.067 0.125 0.175 0.25 0.45 0.848 1.489 2.053 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.067 0.125 0.175 0.25 0.45 0.848 1.489 2.053 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.067 0.125 0.175 0.25 0.45 0.848 1.489 2.053 O.D. 0.067 0.125 0.175 0.25 0.45 0.848 1.489 2.053

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.