Mouse N-Cadherin-2 CDH2 CD325 ELISA Kit PicoKine®

Cadherin-2 (CDH2), also known as neural cadherin (NCAD), is a protein that in humans is encoded by the CDH2 gene. It is a classical cadherin from the cadherin superfamily. This gene is mapped to 18q12.1. Cadherin-2 is expressed in the brain, skeletal and cardiac muscle. Cadherin-2 is commonly found in cancer cells and provides a mechanism for transendothelial migration. It is a calcium dependent cell-cell adhesion glycoprotein comprising five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1737 1604 1707 1747 1698 56.64 3.3% Sample 2 5669 6007 5146 5724 5636 310.87 5.5% Sample 3 22081 20274 22811 21203 21592 950.46 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1737 1604 1707 1747 1698 56.64 3.3% Sample 1 1737 1604 1707 1747 1698 56.64 3.3% Sample 2 5669 6007 5146 5724 5636 310.87 5.5% Sample 2 5669 6007 5146 5724 5636 310.87 5.5% Sample 3 22081 20274 22811 21203 21592 950.46 4.4% Sample 3 22081 20274 22811 21203 21592 950.46 4.4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1737 5669 22081 1767 5460 20442 Standard deviation 74.69 340.14 1236.53 102.48 425.88 1512.7 CV (%) 4.3% 6% 5.6% 5.8% 7.8% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1737 5669 22081 1767 5460 20442 Standard deviation 74.69 340.14 1236.53 102.48 425.88 1512.7 CV (%) 4.3% 6% 5.6% 5.8% 7.8% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1737 5669 22081 1767 5460 20442 Mean (pg/ml) 1737 5669 22081 1767 5460 20442 Standard deviation 74.69 340.14 1236.53 102.48 425.88 1512.7 Standard deviation 74.69 340.14 1236.53 102.48 425.88 1512.7 CV (%) 4.3% 6% 5.6% 5.8% 7.8% 7.4% CV (%) 4.3% 6% 5.6% 5.8% 7.8% 7.4%

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence (PubMed:24952463) . CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.031 0.169 0.2 0.28 0.401 0.66 1.14 1.88 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.031 0.169 0.2 0.28 0.401 0.66 1.14 1.88 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.031 0.169 0.2 0.28 0.401 0.66 1.14 1.88 O.D. 0.031 0.169 0.2 0.28 0.401 0.66 1.14 1.88

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.