Mouse TIMP-2 ELISA Kit PicoKine®

Mouse TIMP-2 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Timp2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

TIMP-2 gen is encoded by 5 exons spanning 83 kb of genomic DNA. TIMP-2 is 83 kilobase pairs (kb) long with exon-intron splicing sites located in preserved positions among the three members of the TIMP family. The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition. The standard product used in this kit is recombinant human TIMP-2 with the molecular mass of 22Kda and 194 amino acid.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 193 212 196 201 200 7.22 3.6% Sample 2 1314 1183 1090 1242 1207 82.06 6.7% Sample 3 4248 4225 4558 4825 4464 246.44 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 193 212 196 201 200 7.22 3.6% Sample 1 193 212 196 201 200 7.22 3.6% Sample 2 1314 1183 1090 1242 1207 82.06 6.7% Sample 2 1314 1183 1090 1242 1207 82.06 6.7% Sample 3 4248 4225 4558 4825 4464 246.44 5.5% Sample 3 4248 4225 4558 4825 4464 246.44 5.5%

Synonyms

Metalloproteinase inhibitor 2; Tissue inhibitor of metalloproteinases 2; TIMP-2; Timp2; Timp-2

Gene Name

TIMP2

Gene ID

21858

UniProt

P25785

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: C27-P220

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Detected in testis, retina, hippocampus and cerebral cortex. .

Applications

ELISA

Field of Research

Angiogenesis, Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Protease Inhibitors, Proteolysis/Ubiquitin, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 193 1314 4248 179 1283 4028 Standard deviation 10.42 67.01 318.6 10.91 84.67 370.57 CV (%) 5.4% 9.2%% 7.5% 6.1% 6.6% 9.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 193 1314 4248 179 1283 4028 Standard deviation 10.42 67.01 318.6 10.91 84.67 370.57 CV (%) 5.4% 9.2%% 7.5% 6.1% 6.6% 9.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 193 1314 4248 179 1283 4028 Mean (pg/ml) 193 1314 4248 179 1283 4028 Standard deviation 10.42 67.01 318.6 10.91 84.67 370.57 Standard deviation 10.42 67.01 318.6 10.91 84.67 370.57 CV (%) 5.4% 9.2%% 7.5% 6.1% 6.6% 9.2% CV (%) 5.4% 9.2%% 7.5% 6.1% 6.6% 9.2%

Sensitivity

<10 pg/mL

Function

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

24328 MW

Specificity

Natural and recombinant mouse TIMP-2

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Metalloproteinase inhibitor 2

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the protease inhibitor I35 (TIMP) family.

Protein Name

Metalloproteinase inhibitor 2

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.034 0.09 0.152 0.269 0.497 0.931 1.489 2.095 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.034 0.09 0.152 0.269 0.497 0.931 1.489 2.095 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.034 0.09 0.152 0.269 0.497 0.931 1.489 2.095 O.D. 0.034 0.09 0.152 0.269 0.497 0.931 1.489 2.095

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.