Mouse DMP-1 ELISA Kit PicoKine®

Dentin matrix acidic phosphorprotein 1 is a protein that in humans is encoded by the DMP1 gene. It belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family of secreted phosphoproteins. This gene is mapped to 4q22.1. This protein, which is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. In undifferentiated osteoblasts, DMP-1 is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation, the protein becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. Mutations in the gene are known to cause autosomal recessive hypophosphatemia, a disease that manifests as rickets and osteomalacia. The gene structure is conserved in mammals.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 279 274 317 271 285 18.55 6.5% Sample 2 1719 1758 1493 1528 1624 115.49 7.1% Sample 3 4479 4588 5236 4685 4747 291.57 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 279 274 317 271 285 18.55 6.5% Sample 1 279 274 317 271 285 18.55 6.5% Sample 2 1719 1758 1493 1528 1624 115.49 7.1% Sample 2 1719 1758 1493 1528 1624 115.49 7.1% Sample 3 4479 4588 5236 4685 4747 291.57 6.1% Sample 3 4479 4588 5236 4685 4747 291.57 6.1%

Cytoskeleton/ECM, Developmental Biology, ECM Proteins, Extracellular Matrix, Mesenchymal Stem Cells, Organogenesis, Signal Transduction, Skeletal Development, Stem Cells, Structures

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 279 1719 4479 263 1713 4336 Standard deviation 17.7% 103.14 344.88 17.6% 130.18 346.88 CV (%) 5.7% 6% 7.7% 6.2% 7.6% 8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 279 1719 4479 263 1713 4336 Standard deviation 17.7% 103.14 344.88 17.6% 130.18 346.88 CV (%) 5.7% 6% 7.7% 6.2% 7.6% 8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 279 1719 4479 263 1713 4336 Mean (pg/ml) 279 1719 4479 263 1713 4336 Standard deviation 17.7% 103.14 344.88 17.6% 130.18 346.88 Standard deviation 17.7% 103.14 344.88 17.6% 130.18 346.88 CV (%) 5.7% 6% 7.7% 6.2% 7.6% 8% CV (%) 5.7% 6% 7.7% 6.2% 7.6% 8%

May have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts, unphosphorylated form acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase it is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Nucleus . Cytoplasm . Secreted, extracellular space, extracellular matrix . In proliferating preosteoblasts it is nuclear, during early maturation stage is cytoplasmic and in mature osteoblast localizes in the mineralized matrix. Export from the nucleus of differentiating osteoblast is triggered by the release of calcium from intracellular stores followed by a massive influx of this pool of calcium into the nucleus (By similarity) . .

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.017 0.049 0.085 0.164 0.304 0.609 1.183 1.974 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.017 0.049 0.085 0.164 0.304 0.609 1.183 1.974 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.017 0.049 0.085 0.164 0.304 0.609 1.183 1.974 O.D. 0.017 0.049 0.085 0.164 0.304 0.609 1.183 1.974

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.