Human REG-4 ELISA Kit PicoKine®

Regenerating islet-derived protein 4 is a protein that in humans is encoded by the REG4 gene. It is mapped to 1p12. REG-4 gene contains 6 exons and that the exon structure is preserved among members of the REG gene family. This gene is able to maintain carbohydrate recognition activity in an acidic environment. REG-4 expression was significantly upregulated by inflammation and tissue injury associated with active Crohn disease and ulcerative colitis, and most of the colorectal tumors overexpressing REG-4 are mucinous tumors or neuroendocrine tumors.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 323 308 321 370 330 23.52 7.1% Sample 2 1987 2009 2089 2112 2049 52.47 2.5% Sample 3 4639 4973 4766 4823 4800 119.93 2.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 323 308 321 370 330 23.52 7.1% Sample 1 323 308 321 370 330 23.52 7.1% Sample 2 1987 2009 2089 2112 2049 52.47 2.5% Sample 2 1987 2009 2089 2112 2049 52.47 2.5% Sample 3 4639 4973 4766 4823 4800 119.93 2.4% Sample 3 4639 4973 4766 4823 4800 119.93 2.4%

Regenerating islet-derived protein 4; REG-4; Gastrointestinal secretory protein; REG-like protein; Regenerating islet-derived protein IV; Reg IV; REG4; GISP, RELP

Highly expressed in the gastrointestinal tract including the duodenum, jejunum, ileum, ileocecum, appendix, descending colon, pancreas and small intestine. Weakly expressed in normal colon and stomach. Strongly expressed in most colorectal tumors than in normal colon. Preferentially expressed in mucinous tumors and in some cases neuro-endocrine tumors. Expressed in mucus-secreting cells and enterocyte-like cells. In small intestine expressed at the basal perinuclear zone of goblet cells. .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 323 1987 4639 327 2178 4730 Standard deviation 14.85 149.02 250.5 16.67 178.59 335.83 CV (%) 4.6% 7.5% 5.4% 7.1%% 8.2% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 323 1987 4639 327 2178 4730 Standard deviation 14.85 149.02 250.5 16.67 178.59 335.83 CV (%) 4.6% 7.5% 5.4% 7.1%% 8.2% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 323 1987 4639 327 2178 4730 Mean (pg/ml) 323 1987 4639 327 2178 4730 Standard deviation 14.85 149.02 250.5 16.67 178.59 335.83 Standard deviation 14.85 149.02 250.5 16.67 178.59 335.83 CV (%) 4.6% 7.5% 5.4% 7.1%% 8.2% 7.1% CV (%) 4.6% 7.5% 5.4% 7.1%% 8.2% 7.1%

Calcium-independent lectin displaying mannose-binding specificity and able to maintain carbohydrate recognition activity in an acidic environment. May be involved in inflammatory and metaplastic responses of the gastrointestinal epithelium. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.023 0.082 0.147 0.272 0.516 1.052 1.82 2.379 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.023 0.082 0.147 0.272 0.516 1.052 1.82 2.379 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.023 0.082 0.147 0.272 0.516 1.052 1.82 2.379 O.D. 0.023 0.082 0.147 0.272 0.516 1.052 1.82 2.379

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.