Human CRLF2/TSLP R ELISA Kit PicoKine®

Human CRLF2/TSLP R ELISA Kit PicoKine® (96 Tests) . Quantitate Human CRLF2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Cytokine receptor-like factor 2, also known as TSLPR or CRL2, is a protein that in humans is encoded by the CRLF2 gene. It is mapped to Xp22.33. The protein encoded by this gene is a receptor for thymic stromal lymphopoietin (TSLP) . Together with the interleukin 7 receptor (IL7R), the encoded protein and TSLP activate STAT3, STAT5, and JAK2 pathways, which control processes such as cell proliferation and development of the hematopoietic system. Two transcript variants encoding different isoforms have been found for this gene. In addition to it, it has been found that rearrangement of CRLF2 and JAK mutation together contribute to leukemogenesis in B-progenitor ALL.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 110 110 114 115 112 2.27 2% Sample 2 661 633 642 685 655 19.92 3% Sample 3 1213 1047 1166 1231 1164 71.73 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 110 110 114 115 112 2.27 2% Sample 1 110 110 114 115 112 2.27 2% Sample 2 661 633 642 685 655 19.92 3% Sample 2 661 633 642 685 655 19.92 3% Sample 3 1213 1047 1166 1231 1164 71.73 6.1% Sample 3 1213 1047 1166 1231 1164 71.73 6.1%

Synonyms

Cytokine receptor-like factor 2; Cytokine receptor-like 2; IL-XR; Thymic stromal lymphopoietin protein receptor; TSLP receptor; CRLF2; CRL2, ILXR, TSLPR

Gene Name

CRLF2

Gene ID

64109

UniProt

Q9HC73

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: G25-K231

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expressed in heart, skeletal muscle, kidney and adult and fetal liver. Primarily expressed in dendrites and monocytes. Weakly expressed in T-cells.

Applications

ELISA

Field of Research

Cytokines, Immunology, Innate Immunity

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 110 661 1213 107 721 1176 Standard deviation 5.17 27.1 73.99 5.24 37.49 88.2 CV (%) 4.7% 4.1% 6.1% 4.9% 5.2% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 110 661 1213 107 721 1176 Standard deviation 5.17 27.1 73.99 5.24 37.49 88.2 CV (%) 4.7% 4.1% 6.1% 4.9% 5.2% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 110 661 1213 107 721 1176 Mean (pg/ml) 110 661 1213 107 721 1176 Standard deviation 5.17 27.1 73.99 5.24 37.49 88.2 Standard deviation 5.17 27.1 73.99 5.24 37.49 88.2 CV (%) 4.7% 4.1% 6.1% 4.9% 5.2% 7.5% CV (%) 4.7% 4.1% 6.1% 4.9% 5.2% 7.5%

Sensitivity

<10 pg/mL

Function

Receptor for thymic stromal lymphopoietin (TSLP) . Forms a functional complex with TSLP and IL7R which is capable of stimulating cell proliferation through activation of STAT3 and STAT5. Also activates JAK2 (By similarity) . Implicated in the development of the hematopoietic system. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

42013 MW

Specificity

Natural and recombinant human CRLF2

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Cytokine receptor-like factor 2

Subcellular Location

Isoform 1: Cell membrane ; Single-pass type I membrane protein .

Sequence Similarities

Belongs to the type I cytokine receptor family. Type 5 subfamily.

Protein Name

Cytokine receptor-like factor 2

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.044 0.117 0.177 0.3 0.513 0.908 1.513 1.94 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.044 0.117 0.177 0.3 0.513 0.908 1.513 1.94 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.044 0.117 0.177 0.3 0.513 0.908 1.513 1.94 O.D. 0.044 0.117 0.177 0.3 0.513 0.908 1.513 1.94

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.