Human Galectin-7/LGALS7 ELISA Kit PicoKine®

Human Galectin-7/LGALS7 ELISA Kit PicoKine® (96 Tests) . Quantitate Human LGALS7 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Galectin-7, also known as GAL7, is a protein that in humans is encoded by the LGALS7 gene. This gene is mapped to 19q13.2. Galectins belong to a family of related beta-galactoside-binding lectins, also referred to as S-type or S-Lac lectins. Members of this family have been implicated in a variety of functions, including growth regulation, cell adhesion, migration, neoplastic transformation, and immune responses. Differential and in situ hybridizations indicate that this lectin is specifically expressed in keratinocytes. It has been found that expression of Galectin-7 was markedly upregulated in corneal epithelium after injury and that exogenous galectin-7 stimulated reepithelialization of corneal wounds.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 185 222 208 223 209 15.33 7.3% Sample 2 1753 1862 1626 1898 1784 106.06 5.9% Sample 3 5922 5367 5932 6244 5866 315.98 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 185 222 208 223 209 15.33 7.3% Sample 1 185 222 208 223 209 15.33 7.3% Sample 2 1753 1862 1626 1898 1784 106.06 5.9% Sample 2 1753 1862 1626 1898 1784 106.06 5.9% Sample 3 5922 5367 5932 6244 5866 315.98 5.3% Sample 3 5922 5367 5932 6244 5866 315.98 5.3%

Synonyms

Galectin-7; Gal-7; HKL-14; PI7; p53-induced gene 1 protein; LGALS7; PIG1; LGALS7B

Gene Name

LGALS7

Gene ID

3963

UniProt

P47929

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S2-F136

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Mainly expressed in stratified squamous epithelium.

Applications

ELISA

Field of Research

Apoptosis, Cancer, Cell Adhesion Proteins, Cell Death, Invasion/Microenvironment, Neuroscience

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 185 1753 5922 182 1745 5903 Standard deviation 13.13 91.15 236.88 14.37 111.68 277.44 CV (%) 7.1% 5.2% 4% 7.9% 6.4% 4.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 185 1753 5922 182 1745 5903 Standard deviation 13.13 91.15 236.88 14.37 111.68 277.44 CV (%) 7.1% 5.2% 4% 7.9% 6.4% 4.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 185 1753 5922 182 1745 5903 Mean (pg/ml) 185 1753 5922 182 1745 5903 Standard deviation 13.13 91.15 236.88 14.37 111.68 277.44 Standard deviation 13.13 91.15 236.88 14.37 111.68 277.44 CV (%) 7.1% 5.2% 4% 7.9% 6.4% 4.7% CV (%) 7.1% 5.2% 4% 7.9% 6.4% 4.7%

Sensitivity

<10 pg/mL

Function

Could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

15075 MW

Specificity

Natural and recombinant human Galectin-7

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Galectin-7

Subcellular Location

Cytoplasm . Nucleus . Secreted . May be secreted by a non-classical secretory pathway.

Sequence Similarities

Contains 1 galectin domain.

Protein Name

Galectin-7

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.044 0.133 0.19 0.309 0.527 0.952 1.482 2.157 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.044 0.133 0.19 0.309 0.527 0.952 1.482 2.157 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.044 0.133 0.19 0.309 0.527 0.952 1.482 2.157 O.D. 0.044 0.133 0.19 0.309 0.527 0.952 1.482 2.157

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.