Human CXCL14/Brak ELISA Kit PicoKine®

Chemokine (C-X-C motif) ligand 14 (CXCL14) is a small cytokine belonging to the CXC chemokine family that is also known as BRAK (for breast and kidney-expressed chemokine) . This gene is mapped to 5q31.1. CXCL14 is constitutively expressed at high levels in many normal tissues, it has been found that fibroblasts are the primary source of CXCL14 and that CXCL14 is involved in the homeostasis of monocyte-derived macrophages rather than in inflammation. It is also a potent chemoattractant and activator of dendritic cells, is implicated in homing of these cells, and can stimulate the migration of activated NK cells. CXCL14 also inhibits angiogenesis, possibly as a result of its ability to block endothelial cell chemotaxis. In addition to it, CXCL14 is a potent chemokine for immature DCs, with a chemoattractive activity comparable to bacterial fMLP and MIP1A.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 174 182 174 200 182 10.61 5.8% Sample 2 718 818 718 802 764 46.34 6% Sample 3 1106 1003 1075 1062 1061 37.36 3.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 174 182 174 200 182 10.61 5.8% Sample 1 174 182 174 200 182 10.61 5.8% Sample 2 718 818 718 802 764 46.34 6% Sample 2 718 818 718 802 764 46.34 6% Sample 3 1106 1003 1075 1062 1061 37.36 3.5% Sample 3 1106 1003 1075 1062 1061 37.36 3.5%

Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highly expressed in normal tissue without inflammatory stimuli and infrequently expressed in cancer cell lines. Weakly expressed in monocyte- derived dendritic cells. Not detected in lung or unstimulated peripheral blood lymphocytes. .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 174 718 1106 165 723 1109 Standard deviation 7.3 53.85 78.52 9.07 64.34 82.06 CV (%) 4.2% 7.5% 7.1% 5.5% 8.9% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 174 718 1106 165 723 1109 Standard deviation 7.3 53.85 78.52 9.07 64.34 82.06 CV (%) 4.2% 7.5% 7.1% 5.5% 8.9% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 174 718 1106 165 723 1109 Mean (pg/ml) 174 718 1106 165 723 1109 Standard deviation 7.3 53.85 78.52 9.07 64.34 82.06 Standard deviation 7.3 53.85 78.52 9.07 64.34 82.06 CV (%) 4.2% 7.5% 7.1% 5.5% 8.9% 7.4% CV (%) 4.2% 7.5% 7.1% 5.5% 8.9% 7.4%

Potent chemoattractant for neutrophils, and weaker for dendritic cells. Not chemotactic for T-cells, B-cells, monocytes, natural killer cells or granulocytes. Does not inhibit proliferation of myeloid progenitors in colony formation assays. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 125 250 500 1000 2000 4000 O.D. 0.087 0.189 0.39 0.789 1.226 1.896 2.419 nan Concentration (pg/ml) 0 125 250 500 1000 2000 4000 O.D. 0.087 0.189 0.39 0.789 1.226 1.896 2.419 nan Concentration (pg/ml) 0 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 O.D. 0.087 0.189 0.39 0.789 1.226 1.896 2.419 nan O.D. 0.087 0.189 0.39 0.789 1.226 1.896 2.419 nan

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.