Human IL-18BP ELISA Kit PicoKine®

Interleukin-18-binding protein, also known as IL18BPa, is a protein that in humans is encoded by the IL18BP gene. It is mapped to 11q13.4. The protein encoded by this gene is an inhibitor of the proinflammatory cytokine IL18. This protein binds to IL18, prevents the binding of IL18 to its receptor, and thus inhibits IL18-induced IFN-gamma production. This protein is constitutively expressed and secreted in mononuclear cells. The expression of this protein can be enhanced by IFN-gamma. It has been found that IL18BP expression in intestinal tissue is increased in endothelial cells as well as cells of the submucosa and overlying lymphoid aggregates in Crohn disease.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 122 128 142 139 132 8.1 6.1% Sample 2 924 885 907 868 896 21.27 2.3% Sample 3 2127 2341 2469 2295 2308 122.41 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 122 128 142 139 132 8.1 6.1% Sample 1 122 128 142 139 132 8.1 6.1% Sample 2 924 885 907 868 896 21.27 2.3% Sample 2 924 885 907 868 896 21.27 2.3% Sample 3 2127 2341 2469 2295 2308 122.41 5.3% Sample 3 2127 2341 2469 2295 2308 122.41 5.3%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 122 924 2127 117 937 2003 Standard deviation 7.07 47.12 110.6 8.89 65.59 110.16 CV (%) 5.8% 5.5% 5.2% 7.6% 7% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 122 924 2127 117 937 2003 Standard deviation 7.07 47.12 110.6 8.89 65.59 110.16 CV (%) 5.8% 5.5% 5.2% 7.6% 7% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 122 924 2127 117 937 2003 Mean (pg/ml) 122 924 2127 117 937 2003 Standard deviation 7.07 47.12 110.6 8.89 65.59 110.16 Standard deviation 7.07 47.12 110.6 8.89 65.59 110.16 CV (%) 5.8% 5.5% 5.2% 7.6% 7% 5.5% CV (%) 5.8% 5.5% 5.2% 7.6% 7% 5.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.02 0.084 0.155 0.277 0.494 0.888 1.52 2.123 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.02 0.084 0.155 0.277 0.494 0.888 1.52 2.123 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.02 0.084 0.155 0.277 0.494 0.888 1.52 2.123 O.D. 0.02 0.084 0.155 0.277 0.494 0.888 1.52 2.123

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.