Human Granulysin ELISA Kit PicoKine®

Granulysin is a substance released by cytotoxic T cells (CD8) when they are attached to infected body cells. The product of this gene is a member of the saposin-like protein (SAPLIP) family. It is mapped to 2p11.2. Granulysin functions to create holes in the target cell membrane and destroy it. It is able to induce apoptosis in target cells and also has antimicrobial action. This gene is expressed in cytolytic granules with perforin, a pore forming protein, and granzymes that are also involved in cytolysis. In addition to it, Granulysin is broadly antimicrobial, killing microbes that cause, for example, tuberculosis and malaria, and can destroy some tumors. A series of peptides generated from the amino acid sequence of Granulysin are potential antibiotics. It has been found that secretory Granulysin is a key molecule responsible for the disseminated keratinocyte death in SJS/TEN.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 57 49 55 50 52 3.34 6.4% Sample 2 258 240 225 222 236 14.28 6% Sample 3 911 774 819 851 838 49.88 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 57 49 55 50 52 3.34 6.4% Sample 1 57 49 55 50 52 3.34 6.4% Sample 2 258 240 225 222 236 14.28 6% Sample 2 258 240 225 222 236 14.28 6% Sample 3 911 774 819 851 838 49.88 5.9% Sample 3 911 774 819 851 838 49.88 5.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 57 258 911 56 266 859 Standard deviation 4.16 12.64 60.12 4.14 13.3 62.7 CV (%) 7.3% 4.9% 6.6% 7.4% 5% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 57 258 911 56 266 859 Standard deviation 4.16 12.64 60.12 4.14 13.3 62.7 CV (%) 7.3% 4.9% 6.6% 7.4% 5% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 57 258 911 56 266 859 Mean (pg/ml) 57 258 911 56 266 859 Standard deviation 4.16 12.64 60.12 4.14 13.3 62.7 Standard deviation 4.16 12.64 60.12 4.14 13.3 62.7 CV (%) 7.3% 4.9% 6.6% 7.4% 5% 7.3% CV (%) 7.3% 4.9% 6.6% 7.4% 5% 7.3%

Antimicrobial protein that kills intracellular pathogens. Active against a broad range of microbes, including Gram-positive and Gram-negative bacteria, fungi, and parasites. Kills Mycobacterium tuberculosis.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.007 0.093 0.165 0.307 0.525 0.93 1.636 2.705 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.007 0.093 0.165 0.307 0.525 0.93 1.636 2.705 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.007 0.093 0.165 0.307 0.525 0.93 1.636 2.705 O.D. 0.007 0.093 0.165 0.307 0.525 0.93 1.636 2.705

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.