Mouse Galectin-7/LGALS7 ELISA Kit PicoKine®

Galectin-7, also known as GAL7, is a protein that in humans is encoded by the LGALS7 gene. This gene is mapped to 19q13.2. Galectins belong to a family of related beta-galactoside-binding lectins, also referred to as S-type or S-Lac lectins. Members of this family have been implicated in a variety of functions, including growth regulation, cell adhesion, migration, neoplastic transformation, and immune responses. Differential and in situ hybridizations indicate that this lectin is specifically expressed in keratinocytes. It has been found that expression of Galectin-7 was markedly upregulated in corneal epithelium after injury and that exogenous galectin-7 stimulated reepithelialization of corneal wounds.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 161 138 155 158 153 8.91 5.8% Sample 2 861 902 914 909 896 20.93 2.3% Sample 3 2074 2175 2110 2264 2155 72.22 3.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 161 138 155 158 153 8.91 5.8% Sample 1 161 138 155 158 153 8.91 5.8% Sample 2 861 902 914 909 896 20.93 2.3% Sample 2 861 902 914 909 896 20.93 2.3% Sample 3 2074 2175 2110 2264 2155 72.22 3.3% Sample 3 2074 2175 2110 2264 2155 72.22 3.3%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 161 861 2074 152 805 2004 Standard deviation 6.6 40.46 163.84 7.44 42.66 168.33 CV (%) 4.1% 4.7% 7.9% 4.9% 5.3% 8.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 161 861 2074 152 805 2004 Standard deviation 6.6 40.46 163.84 7.44 42.66 168.33 CV (%) 4.1% 4.7% 7.9% 4.9% 5.3% 8.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 161 861 2074 152 805 2004 Mean (pg/ml) 161 861 2074 152 805 2004 Standard deviation 6.6 40.46 163.84 7.44 42.66 168.33 Standard deviation 6.6 40.46 163.84 7.44 42.66 168.33 CV (%) 4.1% 4.7% 7.9% 4.9% 5.3% 8.4% CV (%) 4.1% 4.7% 7.9% 4.9% 5.3% 8.4%

Could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.039 0.119 0.193 0.346 0.565 0.945 1.61 2.225 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.039 0.119 0.193 0.346 0.565 0.945 1.61 2.225 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.039 0.119 0.193 0.346 0.565 0.945 1.61 2.225 O.D. 0.039 0.119 0.193 0.346 0.565 0.945 1.61 2.225

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.