Human Galectin-2 ELISA Kit PicoKine®

Human Galectin-2 ELISA Kit PicoKine® (96 Tests) . Quantitate Human LGALS2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Galectin-2 is a protein that in humans is encoded by the LGALS2 gene. It is mapped to 22q13.1. The protein encoded by this gene is a soluble beta-galactoside binding lectin. The encoded protein is found as a homodimer and can bind to lymphotoxin-alpha. It has been found that a single-nucleotide polymorphism in Galectin-2 is significantly associated with susceptibility to myocardial infarction. This genetic substitution affects the transcriptional level of Galectin-2 in vitro, potentially leading to altered secretion of LTA, to which Galectin-2 binds, which would then affect the degree of inflammation.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 288 323 331 326 317 16.98 5.3% Sample 2 1975 1974 1898 1989 1959 35.71 1.8% Sample 3 5128 5263 5282 4554 5056 296.27 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 288 323 331 326 317 16.98 5.3% Sample 1 288 323 331 326 317 16.98 5.3% Sample 2 1975 1974 1898 1989 1959 35.71 1.8% Sample 2 1975 1974 1898 1989 1959 35.71 1.8% Sample 3 5128 5263 5282 4554 5056 296.27 5.8% Sample 3 5128 5263 5282 4554 5056 296.27 5.8%

Synonyms

Galectin-2; Gal-2; Beta-galactoside-binding lectin L-14-II; HL14; Lactose-binding lectin 2; S-Lac lectin 2; LGALS2

Gene Name

LGALS2

Gene ID

3957

UniProt

P05162

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: M1-E132

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cell Adhesion Proteins, Cell Biology, Cell Cycle, Cell Differentiation, Neuroscience

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 288 1975 5128 263 1848 5204 Standard deviation 21.31 100.72 302.55 23.4 129.36 369.48 CV (%) 7.4% 7.1% 5.9% 8.9% 7% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 288 1975 5128 263 1848 5204 Standard deviation 21.31 100.72 302.55 23.4 129.36 369.48 CV (%) 7.4% 7.1% 5.9% 8.9% 7% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 288 1975 5128 263 1848 5204 Mean (pg/ml) 288 1975 5128 263 1848 5204 Standard deviation 21.31 100.72 302.55 23.4 129.36 369.48 Standard deviation 21.31 100.72 302.55 23.4 129.36 369.48 CV (%) 7.4% 7.1% 5.9% 8.9% 7% 7.1% CV (%) 7.4% 7.1% 5.9% 8.9% 7% 7.1%

Sensitivity

<10 pg/mL

Function

This protein binds beta-galactoside. Its physiological function is not yet known.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

14644 MW

Specificity

Natural and recombinant human Galectin-2

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Galectin-2

Sequence Similarities

Contains 1 galectin domain.

Protein Name

Galectin-2

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.019 0.163 0.261 0.406 0.692 1.098 1.682 1.981 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.019 0.163 0.261 0.406 0.692 1.098 1.682 1.981 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.019 0.163 0.261 0.406 0.692 1.098 1.682 1.981 O.D. 0.019 0.163 0.261 0.406 0.692 1.098 1.682 1.981

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.