Human Galectin-2 ELISA Kit PicoKine®

Galectin-2 is a protein that in humans is encoded by the LGALS2 gene. It is mapped to 22q13.1. The protein encoded by this gene is a soluble beta-galactoside binding lectin. The encoded protein is found as a homodimer and can bind to lymphotoxin-alpha. It has been found that a single-nucleotide polymorphism in Galectin-2 is significantly associated with susceptibility to myocardial infarction. This genetic substitution affects the transcriptional level of Galectin-2 in vitro, potentially leading to altered secretion of LTA, to which Galectin-2 binds, which would then affect the degree of inflammation.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 288 323 331 326 317 16.98 5.3% Sample 2 1975 1974 1898 1989 1959 35.71 1.8% Sample 3 5128 5263 5282 4554 5056 296.27 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 288 323 331 326 317 16.98 5.3% Sample 1 288 323 331 326 317 16.98 5.3% Sample 2 1975 1974 1898 1989 1959 35.71 1.8% Sample 2 1975 1974 1898 1989 1959 35.71 1.8% Sample 3 5128 5263 5282 4554 5056 296.27 5.8% Sample 3 5128 5263 5282 4554 5056 296.27 5.8%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 288 1975 5128 263 1848 5204 Standard deviation 21.31 100.72 302.55 23.4 129.36 369.48 CV (%) 7.4% 7.1% 5.9% 8.9% 7% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 288 1975 5128 263 1848 5204 Standard deviation 21.31 100.72 302.55 23.4 129.36 369.48 CV (%) 7.4% 7.1% 5.9% 8.9% 7% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 288 1975 5128 263 1848 5204 Mean (pg/ml) 288 1975 5128 263 1848 5204 Standard deviation 21.31 100.72 302.55 23.4 129.36 369.48 Standard deviation 21.31 100.72 302.55 23.4 129.36 369.48 CV (%) 7.4% 7.1% 5.9% 8.9% 7% 7.1% CV (%) 7.4% 7.1% 5.9% 8.9% 7% 7.1%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.019 0.163 0.261 0.406 0.692 1.098 1.682 1.981 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.019 0.163 0.261 0.406 0.692 1.098 1.682 1.981 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.019 0.163 0.261 0.406 0.692 1.098 1.682 1.981 O.D. 0.019 0.163 0.261 0.406 0.692 1.098 1.682 1.981

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.