Mouse DLL4 ELISA Kit PicoKine®

Mouse DLL4 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Dll4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Delta like ligand 4 is a protein that in humans is encoded by the DLL4 gene. It is mapped to 15q15.1. This gene is a homolog of the Drosophila delta gene. DLL4 is a transmembrane ligand for Notch receptors that shows restricted expression to endothelial cells (ECs), in particular to arteries and capillaries, and is involved in vascular development, and it also appeared to be a major trigger of Notch receptor activities previously implicated in arterial and vascular development. Mouse DLL4 could activate mouse Notch1 and mouse Notch4. DLL4 acts as a negative regulator of tumor angiogenesis, its blockade results in the striking uncoupling of tumor growth from vessel density, presenting a novel therapeutic approach even for tumors resistant to anti-VEGF therapies. In addition to it, this gene also plays an important role in promoting Th17 effector activity during mycobacterial challenge.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 40 42 36 40 39 2.17 5.5% Sample 2 244 258 252 224 244 12.83 5.2% Sample 3 515 528 493 536 518 16.26 3.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 40 42 36 40 39 2.17 5.5% Sample 1 40 42 36 40 39 2.17 5.5% Sample 2 244 258 252 224 244 12.83 5.2% Sample 2 244 258 252 224 244 12.83 5.2% Sample 3 515 528 493 536 518 16.26 3.1% Sample 3 515 528 493 536 518 16.26 3.1%

Synonyms

Delta-like protein 4; Drosophila Delta homolog 4; Delta4; Dll4

Gene Name

DLL4

Gene ID

54485

UniProt

Q9JI71

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S28-P525

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expressed in vascular endothelium. Expressed in retina at least during embryogenesis. .

Applications

ELISA

Field of Research

Cell Biology, Cell Cycle, Cell Differentiation, Epigenetics and Nuclear Signaling, Neurology Process, Neuroscience

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 40 244 515 40 242 520 Standard deviation 2.44 18.78 36.56 2.64 20.81 38.48 CV (%) 6.1% 7.7% 7.1% 6.6% 8.6% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 40 244 515 40 242 520 Standard deviation 2.44 18.78 36.56 2.64 20.81 38.48 CV (%) 6.1% 7.7% 7.1% 6.6% 8.6% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 40 244 515 40 242 520 Mean (pg/ml) 40 244 515 40 242 520 Standard deviation 2.44 18.78 36.56 2.64 20.81 38.48 Standard deviation 2.44 18.78 36.56 2.64 20.81 38.48 CV (%) 6.1% 7.7% 7.1% 6.6% 8.6% 7.4% CV (%) 6.1% 7.7% 7.1% 6.6% 8.6% 7.4%

Sensitivity

<10 pg/mL

Function

Involved in the Notch signaling pathway as Notch ligand. Activates NOTCH1 and NOTCH4. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Essential for retinal progenitor proliferation is required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types. During spinal cord neurogenesis, inhibits V2a interneuron fate. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

74991 MW

Specificity

Natural and recombinant mouse DLL4

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Delta-like protein 4

Subcellular Location

Membrane ; Single-pass type I membrane protein .

Sequence Similarities

Contains 1 DSL domain.

Protein Name

Delta-like protein 4

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.065 0.126 0.166 0.263 0.455 0.78 1.405 2.088 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.065 0.126 0.166 0.263 0.455 0.78 1.405 2.088 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.065 0.126 0.166 0.263 0.455 0.78 1.405 2.088 O.D. 0.065 0.126 0.166 0.263 0.455 0.78 1.405 2.088

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.