Human Galectin-4 ELISA Kit PicoKine®

Human Galectin-4 ELISA Kit PicoKine® (96 Tests) . Quantitate Human LGALS4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Galectin-4, also known as LGALS4 or GAL4, is a protein that in humans is encoded by the LGALS4 gene. It is mapped to 19q13.2. Galectins are a family of animal lectins defined by an affinity for beta-galactoside-containing saccharides and by shared sequence elements. Galectin-4 is expressed as a 38-kD protein in the human colon adenocarcinoma T84 cell line. Galectin-4 is an S-type lectin that is strongly underexpressed in colorectal cancer, and this gene plays a role in cell adhesion. It has been found that decreased Galectin-4 expression occurs early in colorectal carcinogenesis.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 60 54 60 64 59 3.57 6% Sample 2 243 258 258 260 254 6.83 2.6% Sample 3 879 923 848 978 907 48.89 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 60 54 60 64 59 3.57 6% Sample 1 60 54 60 64 59 3.57 6% Sample 2 243 258 258 260 254 6.83 2.6% Sample 2 243 258 258 260 254 6.83 2.6% Sample 3 879 923 848 978 907 48.89 5.3% Sample 3 879 923 848 978 907 48.89 5.3%

Synonyms

Galectin-4; Gal-4; Antigen NY-CO-27; L-36 lactose-binding protein; L36LBP; Lactose-binding lectin 4; LGALS4

Gene Name

LGALS4

Gene ID

3960

UniProt

P56470

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A2-I323

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cancer, Cell Adhesion,2339, Cytoskeleton/ECM, Energy Metabolism, Energy Transfer Pathways, Epigenetics and Nuclear Signaling, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction, Tags & Cell Markers, Transcription, Transcription Factors

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 60 243 879 64 223 865 Standard deviation 3.24 19.19 45.7 4.28 19.4 47.57 CV (%) 5.4% 7.9% 5.2% 6.7% 8.7% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 60 243 879 64 223 865 Standard deviation 3.24 19.19 45.7 4.28 19.4 47.57 CV (%) 5.4% 7.9% 5.2% 6.7% 8.7% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 60 243 879 64 223 865 Mean (pg/ml) 60 243 879 64 223 865 Standard deviation 3.24 19.19 45.7 4.28 19.4 47.57 Standard deviation 3.24 19.19 45.7 4.28 19.4 47.57 CV (%) 5.4% 7.9% 5.2% 6.7% 8.7% 5.5% CV (%) 5.4% 7.9% 5.2% 6.7% 8.7% 5.5%

Sensitivity

<10 pg/mL

Function

Galectin that binds lactose and a related range of sugars. May be involved in the assembly of adherens junctions.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

35941 MW

Specificity

Natural and recombinant human Galectin-4

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Galectin-4

Sequence Similarities

Contains 2 galectin domains.

Protein Name

Galectin-4

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.067 0.125 0.18 0.283 0.482 0.847 1.568 2.589 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.067 0.125 0.18 0.283 0.482 0.847 1.568 2.589 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.067 0.125 0.18 0.283 0.482 0.847 1.568 2.589 O.D. 0.067 0.125 0.18 0.283 0.482 0.847 1.568 2.589

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.