Mouse LBP ELISA Kit PicoKine®

Lipopolysaccharide binding protein is a protein that in humans is encoded by the LBP gene. This gene is mapped to 20q11.23. LBP is a soluble acute-phase protein that binds to bacterial lipopolysaccharide (or LPS) to elicit immune responses by presenting the LPS to important cell surface pattern recognition receptors called CD14 and TLR4. It is present in the cerebrospinal fluid of patients with pneumococcal meningitis. The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. LBP is made in the liver during the acute phase of infections and is thought to function as a carrier for LPS and to help control LPS-dependent monocyte responses.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 3313 3708 3123 3191 3333 226.54 6.7% Sample 2 12997 12936 11430 12001 12341 657.62 5.3% Sample 3 39089 41588 44126 37423 40556 2538.58 6.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 3313 3708 3123 3191 3333 226.54 6.7% Sample 1 3313 3708 3123 3191 3333 226.54 6.7% Sample 2 12997 12936 11430 12001 12341 657.62 5.3% Sample 2 12997 12936 11430 12001 12341 657.62 5.3% Sample 3 39089 41588 44126 37423 40556 2538.58 6.2% Sample 3 39089 41588 44126 37423 40556 2538.58 6.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 3313 12997 39089 3385 14212 39495 Standard deviation 237.12 831.8 2110.8 243.72 1151.17 2804.14 CV (%) 7.1% 6.4% 5.4% 7.2% 8.1% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 3313 12997 39089 3385 14212 39495 Standard deviation 237.12 831.8 2110.8 243.72 1151.17 2804.14 CV (%) 7.1% 6.4% 5.4% 7.2% 8.1% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 3313 12997 39089 3385 14212 39495 Mean (pg/ml) 3313 12997 39089 3385 14212 39495 Standard deviation 237.12 831.8 2110.8 243.72 1151.17 2804.14 Standard deviation 237.12 831.8 2110.8 243.72 1151.17 2804.14 CV (%) 7.1% 6.4% 5.4% 7.2% 8.1% 7.1% CV (%) 7.1% 6.4% 5.4% 7.2% 8.1% 7.1%

Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria, and acts as an affinity enhancer for CD14, facilitating its association with LPS. Promotes the release of cytokines in response to bacterial lipopolysaccharide (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.01 0.073 0.138 0.243 0.484 0.907 1.475 2.004 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.01 0.073 0.138 0.243 0.484 0.907 1.475 2.004 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.01 0.073 0.138 0.243 0.484 0.907 1.475 2.004 O.D. 0.01 0.073 0.138 0.243 0.484 0.907 1.475 2.004

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.