Human Prostasin ELISA Kit PicoKine®

Prostasin is a protein that in humans is encoded by the PRSS8 gene. It is a single-copy gene and mapped to 16p11.2. Prostasin is likely to be the more physiologically relevant protease. This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. Coexpression of Prostasin with either Xenopus or rat ENaC in Xenopus oocytes resulted in a 60% to 80% increase in amiloride-sensitive sodium currents, and that the addition of aprotinin, a serine protease inhibitor, completely prevented this activation. This enzyme is highly expressed in prostate epithelia and is one of several proteolytic enzymes found in seminal fluid. The proprotein is cleaved to produce a light chain and a heavy chain which are associated by a disulfide bond. It is active on peptide linkages involving the carboxyl group of lysine or arginine.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 267 199 286 271 256 15.68 6.1% Sample 2 362 319 570 544 449 18.71 4.2% Sample 3 3489 4836 3635 4078 4010 198.87 5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 267 199 286 271 256 15.68 6.1% Sample 1 267 199 286 271 256 15.68 6.1% Sample 2 362 319 570 544 449 18.71 4.2% Sample 2 362 319 570 544 449 18.71 4.2% Sample 3 3489 4836 3635 4078 4010 198.87 5% Sample 3 3489 4836 3635 4078 4010 198.87 5%

Found in prostate, liver, salivary gland, kidney, lung, pancreas, colon, bronchus and renal proximal tubular cells. In the prostate gland it may be synthesized in epithelial cells, secreted into the ducts, and excreted into the seminal fluid.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 267 577 3816 239 418 3890 Standard deviation 12.04 18.93 247.17 16.37 19.65 237.21 CV (%) 4.5% 3.3% 6.3% 6.9% 4.7% 6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 267 577 3816 239 418 3890 Standard deviation 12.04 18.93 247.17 16.37 19.65 237.21 CV (%) 4.5% 3.3% 6.3% 6.9% 4.7% 6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 267 577 3816 239 418 3890 Mean (pg/ml) 267 577 3816 239 418 3890 Standard deviation 12.04 18.93 247.17 16.37 19.65 237.21 Standard deviation 12.04 18.93 247.17 16.37 19.65 237.21 CV (%) 4.5% 3.3% 6.3% 6.9% 4.7% 6% CV (%) 4.5% 3.3% 6.3% 6.9% 4.7% 6%

Possesses a trypsin-like cleavage specificity with a preference for poly-basic substrates. Stimulates epithelial sodium channel (ENaC) activity through activating cleavage of the gamma subunits (SCNN1G) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.035 0.096 0.16 0.251 0.432 0.763 1.289 1.839 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.035 0.096 0.16 0.251 0.432 0.763 1.289 1.839 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.035 0.096 0.16 0.251 0.432 0.763 1.289 1.839 O.D. 0.035 0.096 0.16 0.251 0.432 0.763 1.289 1.839

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.