Human IL-18R1 ELISA Kit PicoKine®

The interleukin-18 receptor 1 (IL-18R1), also known as CDw218a (cluster of differentiation w218a) or IL18RA, is an interleukin receptor of the immunoglobulin superfamily. The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This gene along with four other members of the interleukin 1 receptor family, including IL1R2, IL1R1, ILRL2 (IL-1Rrp2), and IL1RL1 (T1/ST2), form a gene cluster on chromosome 2q. This receptor specifically binds interleukin 18 (IL18), and is essential for IL18 mediated signal transduction. IFN-alpha and IL12 are reported to induce the expression of this receptor in NK and T cells.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 73 73 69 77 73 2.82 3.8% Sample 2 260 269 256 285 267 11.14 4.1% Sample 3 905 862 955 814 884 52.12 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 73 73 69 77 73 2.82 3.8% Sample 1 73 73 69 77 73 2.82 3.8% Sample 2 260 269 256 285 267 11.14 4.1% Sample 2 260 269 256 285 267 11.14 4.1% Sample 3 905 862 955 814 884 52.12 5.8% Sample 3 905 862 955 814 884 52.12 5.8%

Interleukin-18 receptor 1; IL-18R-1; IL-18R1; CD218 antigen-like family member A; CDw218a; IL1 receptor-related protein; IL-1Rrp; IL1R-rp; CD218a; IL18R1; IL1RRP

Expressed in lung, leukocytes, spleen, liver, thymus, prostate, small intestine, colon, placenta, and heart, and is absent from brain, skeletal muscle, pancreas, and kidney. High level of expression in Hodgkin disease cell lines.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 73 260 905 69 279 830 Standard deviation 7.25 11.96 57.20 7.37 17.85 60.59 CV (%) 7.2% 4.6% 6.1% 7.5% 6.4% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 73 260 905 69 279 830 Standard deviation 7.25 11.96 57.20 7.37 17.85 60.59 CV (%) 7.2% 4.6% 6.1% 7.5% 6.4% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 73 260 905 69 279 830 Mean (pg/ml) 73 260 905 69 279 830 Standard deviation 7.25 11.96 57.20 7.37 17.85 60.59 Standard deviation 7.25 11.96 57.20 7.37 17.85 60.59 CV (%) 7.2% 4.6% 6.1% 7.5% 6.4% 7.3% CV (%) 7.2% 4.6% 6.1% 7.5% 6.4% 7.3%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.032 0.133 0.203 0.305 0.468 0.806 1.349 2.031 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.032 0.133 0.203 0.305 0.468 0.806 1.349 2.031 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.032 0.133 0.203 0.305 0.468 0.806 1.349 2.031 O.D. 0.032 0.133 0.203 0.305 0.468 0.806 1.349 2.031

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.