Human Ficolin-2 ELISA Kit PicoKine®

Ficolin-2, which was initially identified as L-ficolin, is a protein that in humans is encoded by the FCN2 gene. The product of this gene belongs to the ficolin family of proteins. This family is characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. It is mapped to 9q34.3. This gene is predominantly expressed in the liver, and has been shown to have carbohydrate binding and opsonic activities. Serum concentration of ficolin-2 varies considerably in healthy individuals. FCN2 promoter polymorphisms were associated with marked changes in theficolin-2 serum concentration.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 977 1058 1110 1008 1038 50.5 4.8% Sample 2 6812 6337 6515 7162 6706 312.97 4.6% Sample 3 22201 22603 22446 21704 22238 340.22 1.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 977 1058 1110 1008 1038 50.5 4.8% Sample 1 977 1058 1110 1008 1038 50.5 4.8% Sample 2 6812 6337 6515 7162 6706 312.97 4.6% Sample 2 6812 6337 6515 7162 6706 312.97 4.6% Sample 3 22201 22603 22446 21704 22238 340.22 1.5% Sample 3 22201 22603 22446 21704 22238 340.22 1.5%

Ficolin-2;37 kDa elastin-binding protein; Collagen/fibrinogen domain-containing protein 2; EBP-37; Ficolin-B; Ficolin-beta; Hucolin; L-ficolin; Serum lectin p35; FCN2; FCNL

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 977 6812 22201 979 6528 20934 Standard deviation 44.94 497.27 1354.26 52.86 509.18 1611.91 CV (%) 4.6% 7.3% 6.1% 5.4% 7.8% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 977 6812 22201 979 6528 20934 Standard deviation 44.94 497.27 1354.26 52.86 509.18 1611.91 CV (%) 4.6% 7.3% 6.1% 5.4% 7.8% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 977 6812 22201 979 6528 20934 Mean (pg/ml) 977 6812 22201 979 6528 20934 Standard deviation 44.94 497.27 1354.26 52.86 509.18 1611.91 Standard deviation 44.94 497.27 1354.26 52.86 509.18 1611.91 CV (%) 4.6% 7.3% 6.1% 5.4% 7.8% 7.7% CV (%) 4.6% 7.3% 6.1% 5.4% 7.8% 7.7%

May function in innate immunity through activation of the lectin complement pathway. Calcium-dependent and GlcNAc- binding lectin. Enhances phagocytosis of S.typhimurium by neutrophils, suggesting an opsonic effect via the collagen region. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.01 0.089 0.136 0.243 0.464 0.862 1.376 2.2 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.01 0.089 0.136 0.243 0.464 0.862 1.376 2.2 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.01 0.089 0.136 0.243 0.464 0.862 1.376 2.2 O.D. 0.01 0.089 0.136 0.243 0.464 0.862 1.376 2.2

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.