Human Ficolin-1 / FCN1 / M Ficolin ELISA Kit PicoKine®

Ficolin-1, and also commonly termed M-ficolin is a protein that in humans is encoded by the FCN1 gene. It is mapped to 9q34.3. The ficolin family of proteins are characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. Ficolins have a crucial role in defense against pneumococcal infection through the lectin complement pathway. Ficolin 1 is predominantly expressed in the peripheral blood leukocytes, and has been postulated to function as a plasma protein with elastin-binding activity.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1355 1460 1601 1587 1500 100.48 6.6% Sample 2 8333 7592 7727 7941 7898 280.15 3.5% Sample 3 19299 22441 21939 20805 21121 1207.37 5.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1355 1460 1601 1587 1500 100.48 6.6% Sample 1 1355 1460 1601 1587 1500 100.48 6.6% Sample 2 8333 7592 7727 7941 7898 280.15 3.5% Sample 2 8333 7592 7727 7941 7898 280.15 3.5% Sample 3 19299 22441 21939 20805 21121 1207.37 5.7% Sample 3 19299 22441 21939 20805 21121 1207.37 5.7%

Peripheral blood leukocytes, monocytes and granulocytes. Also detected in spleen, lung, and thymus, may be due to the presence of tissue macrophages or trapped blood in these tissues. Not detected on lymphocytes. .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1355 8333 19299 1365 8931 19839 Standard deviation 88.07 483.31 1042.14 95.55 544.79 1091.14 CV (%) 6.5% 5.8% 5.4% 7% 6.1% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1355 8333 19299 1365 8931 19839 Standard deviation 88.07 483.31 1042.14 95.55 544.79 1091.14 CV (%) 6.5% 5.8% 5.4% 7% 6.1% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1355 8333 19299 1365 8931 19839 Mean (pg/ml) 1355 8333 19299 1365 8931 19839 Standard deviation 88.07 483.31 1042.14 95.55 544.79 1091.14 Standard deviation 88.07 483.31 1042.14 95.55 544.79 1091.14 CV (%) 6.5% 5.8% 5.4% 7% 6.1% 5.5% CV (%) 6.5% 5.8% 5.4% 7% 6.1% 5.5%

Extracellular lectin functioning as a pattern- recognition receptor in innate immunity. Binds the sugar moieties of pathogen-associated molecular patterns (PAMPs) displayed on microbes and activates the lectin pathway of the complement system. May also activate monocytes through a G protein-coupled receptor, FFAR2, inducing the secretion of interleukin-8/IL-8 (PubMed:21037097) . Binds preferentially to 9-O-acetylated 2-6- linked sialic acid derivatives and to various glycans containing sialic acid engaged in a 2-3 linkage. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.01 0.079 0.153 0.276 0.528 1.017 1.47 2.047 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.01 0.079 0.153 0.276 0.528 1.017 1.47 2.047 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.01 0.079 0.153 0.276 0.528 1.017 1.47 2.047 O.D. 0.01 0.079 0.153 0.276 0.528 1.017 1.47 2.047

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.