Human Tetranectin/CLEC3B ELISA Kit PicoKine®

Tetranectin, also called TNA, is a protein that in humans is encoded by the CLEC3B gene. It is mapped to 3p21.31. Tetranectin, a tetrameric protein isolated from human plasma, has 4 identical and noncovalently bound polypeptide chains, each of 181 amino acid residues. It has a specific binding affinity for sulfated polysaccharides and the kringle 4 of plasminogen. The plasma concentration of tetranectin is reduced in patients with various malignancies. Tetranectin is a plasminogen-binding protein that is induced during the mineralization phase of osteogenesis. Thus, tetranectin is a candidate gene for human disorders affecting bone and connective tissue.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 427 481 500 446 463 28.62 6.1% Sample 2 2683 2751 2924 3083 2860 155.75 5.4% Sample 3 8208 9087 8514 7745 8388 487.42 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 427 481 500 446 463 28.62 6.1% Sample 1 427 481 500 446 463 28.62 6.1% Sample 2 2683 2751 2924 3083 2860 155.75 5.4% Sample 2 2683 2751 2924 3083 2860 155.75 5.4% Sample 3 8208 9087 8514 7745 8388 487.42 5.8% Sample 3 8208 9087 8514 7745 8388 487.42 5.8%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 427 2683 8208 405 2547 7804 Standard deviation 28.6 107.32 4632.45 32.4 150.27 561.88 CV (%) 6.7% 4% 5.7% 8% 5.9% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 427 2683 8208 405 2547 7804 Standard deviation 28.6 107.32 4632.45 32.4 150.27 561.88 CV (%) 6.7% 4% 5.7% 8% 5.9% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 427 2683 8208 405 2547 7804 Mean (pg/ml) 427 2683 8208 405 2547 7804 Standard deviation 28.6 107.32 4632.45 32.4 150.27 561.88 Standard deviation 28.6 107.32 4632.45 32.4 150.27 561.88 CV (%) 6.7% 4% 5.7% 8% 5.9% 7.2% CV (%) 6.7% 4% 5.7% 8% 5.9% 7.2%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.048 0.114 0.164 0.275 0.503 0.881 1.605 2.389 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.048 0.114 0.164 0.275 0.503 0.881 1.605 2.389 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.048 0.114 0.164 0.275 0.503 0.881 1.605 2.389 O.D. 0.048 0.114 0.164 0.275 0.503 0.881 1.605 2.389

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.