Human Tetranectin/CLEC3B ELISA Kit PicoKine®

Human Tetranectin/CLEC3B ELISA Kit PicoKine® (96 Tests) . Quantitate Human CLEC3B in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Tetranectin, also called TNA, is a protein that in humans is encoded by the CLEC3B gene. It is mapped to 3p21.31. Tetranectin, a tetrameric protein isolated from human plasma, has 4 identical and noncovalently bound polypeptide chains, each of 181 amino acid residues. It has a specific binding affinity for sulfated polysaccharides and the kringle 4 of plasminogen. The plasma concentration of tetranectin is reduced in patients with various malignancies. Tetranectin is a plasminogen-binding protein that is induced during the mineralization phase of osteogenesis. Thus, tetranectin is a candidate gene for human disorders affecting bone and connective tissue.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 427 481 500 446 463 28.62 6.1% Sample 2 2683 2751 2924 3083 2860 155.75 5.4% Sample 3 8208 9087 8514 7745 8388 487.42 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 427 481 500 446 463 28.62 6.1% Sample 1 427 481 500 446 463 28.62 6.1% Sample 2 2683 2751 2924 3083 2860 155.75 5.4% Sample 2 2683 2751 2924 3083 2860 155.75 5.4% Sample 3 8208 9087 8514 7745 8388 487.42 5.8% Sample 3 8208 9087 8514 7745 8388 487.42 5.8%

Synonyms

Tetranectin; TN; C-type lectin domain family 3 member B; Plasminogen kringle 4-binding protein; CLEC3B; TNA

Gene Name

CLEC3B

Gene ID

7123

UniProt

P05452

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E22-V202

Capture Antibody

Polyclonal antibody from sheep

Detection Antibody

Polyclonal antibody from sheep

Tissue Specificity

Found in plasma.

Applications

ELISA

Field of Research

Cardiovascular, Cytoskeleton/ECM, Extracellular Matrix, Serum Proteins, Signal Transduction, Structures

Standard

20 ng/Tube

Sample Type

Cell culture supernatants and serum.

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 427 2683 8208 405 2547 7804 Standard deviation 28.6 107.32 4632.45 32.4 150.27 561.88 CV (%) 6.7% 4% 5.7% 8% 5.9% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 427 2683 8208 405 2547 7804 Standard deviation 28.6 107.32 4632.45 32.4 150.27 561.88 CV (%) 6.7% 4% 5.7% 8% 5.9% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 427 2683 8208 405 2547 7804 Mean (pg/ml) 427 2683 8208 405 2547 7804 Standard deviation 28.6 107.32 4632.45 32.4 150.27 561.88 Standard deviation 28.6 107.32 4632.45 32.4 150.27 561.88 CV (%) 6.7% 4% 5.7% 8% 5.9% 7.2% CV (%) 6.7% 4% 5.7% 8% 5.9% 7.2%

Sensitivity

<10 pg/mL

Function

Tetranectin binds to plasminogen and to isolated kringle 4. May be involved in the packaging of molecules destined for exocytosis.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

22537 MW

Specificity

Natural and recombinant human Tetranectin

Gene Name Synonym

Tetranectin

Subcellular Location

Secreted.

Sequence Similarities

Contains 1 C-type lectin domain.

Protein Name

Tetranectin

Sample Data

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.048 0.114 0.164 0.275 0.503 0.881 1.605 2.389 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.048 0.114 0.164 0.275 0.503 0.881 1.605 2.389 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.048 0.114 0.164 0.275 0.503 0.881 1.605 2.389 O.D. 0.048 0.114 0.164 0.275 0.503 0.881 1.605 2.389

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.