Mouse Renin 1/Ren1 ELISA Kit PicoKine®

Mouse Renin 1/Ren1 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Ren1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Renin-1 is also known as Ren, Angiotensinogenase or Kidney rennin. It is a member of peptidase A1 family. Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. It associated to membranes via binding to ATP6AP2.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 158 161 142 150 152 7.39 4.8% Sample 2 449 465 437 476 456 14.9 3.2% Sample 3 1246 1215 1179 1158 1199 33.7 2.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 158 161 142 150 152 7.39 4.8% Sample 1 158 161 142 150 152 7.39 4.8% Sample 2 449 465 437 476 456 14.9 3.2% Sample 2 449 465 437 476 456 14.9 3.2% Sample 3 1246 1215 1179 1158 1199 33.7 2.8% Sample 3 1246 1215 1179 1158 1199 33.7 2.8%

Synonyms

Renin-1;3.4.23.15; Angiotensinogenase; Kidney renin; Ren1; Ren, Ren-1

Gene Name

REN

Gene ID

19701

UniProt

P06281

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S72-R402

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Kidney.

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 158 449 1246 147 482 1262 Standard deviation 10.74 28.73 64.79 11.9 34.22 70.67 CV (%) 6.8% 6.4% 5.2% 8.1% 7.1% 5.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 158 449 1246 147 482 1262 Standard deviation 10.74 28.73 64.79 11.9 34.22 70.67 CV (%) 6.8% 6.4% 5.2% 8.1% 7.1% 5.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 158 449 1246 147 482 1262 Mean (pg/ml) 158 449 1246 147 482 1262 Standard deviation 10.74 28.73 64.79 11.9 34.22 70.67 Standard deviation 10.74 28.73 64.79 11.9 34.22 70.67 CV (%) 6.8% 6.4% 5.2% 8.1% 7.1% 5.6% CV (%) 6.8% 6.4% 5.2% 8.1% 7.1% 5.6%

Sensitivity

<10 pg/mL

Function

Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

44343 MW

Specificity

Natural and recombinant mouse Renin-1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Renin-1

Subcellular Location

Secreted . Membrane . Associated to membranes via binding to ATP6AP2. .

Protein Name

Renin-1

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.003 0.137 0.248 0.486 0.777 1.26 1.704 1.925 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.003 0.137 0.248 0.486 0.777 1.26 1.704 1.925 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.003 0.137 0.248 0.486 0.777 1.26 1.704 1.925 O.D. 0.003 0.137 0.248 0.486 0.777 1.26 1.704 1.925

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.