Human Renin / Prorenin ELISA Kit PicoKine®

Renin, also known as an angiotensinogenase, is an enzyme that participates in the body's renin-angiotensin system (RAS) . This gene is mapped to 1q32.1. Renin's primary function is to cause an increase in blood pressure, leading to restoration of perfusion pressure in the kidneys. It also can catalyze the first step in the activation pathway of angiotensinogen--a cascade that can result in aldosterone release, vasoconstriction. Renin, an aspartyl protease, cleaves angiotensinogen to form angiotensin I, which is converted to angiotensin II by angiotensin I converting enzyme, an important regulator of blood pressure and electrolyte balance. What's more, Renin secretion is also stimulated by sympathetic nervous stimulation, mainly through beta-1 adrenoceptor activation.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 73 79 69 73 73 3.57 4.8% Sample 2 224 224 208 232 222 8.71 3.9% Sample 3 875 929 953 900 914 29.42 3.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 73 79 69 73 73 3.57 4.8% Sample 1 73 79 69 73 73 3.57 4.8% Sample 2 224 224 208 232 222 8.71 3.9% Sample 2 224 224 208 232 222 8.71 3.9% Sample 3 875 929 953 900 914 29.42 3.2% Sample 3 875 929 953 900 914 29.42 3.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 73 224 875 68 211 909 Standard deviation 4.52 13.66 56 4.69 16.88 71.81 CV (%) 6.2% 6.1% 6.4% 6.9% 8% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 73 224 875 68 211 909 Standard deviation 4.52 13.66 56 4.69 16.88 71.81 CV (%) 6.2% 6.1% 6.4% 6.9% 8% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 73 224 875 68 211 909 Mean (pg/ml) 73 224 875 68 211 909 Standard deviation 4.52 13.66 56 4.69 16.88 71.81 Standard deviation 4.52 13.66 56 4.69 16.88 71.81 CV (%) 6.2% 6.1% 6.4% 6.9% 8% 7.9% CV (%) 6.2% 6.1% 6.4% 6.9% 8% 7.9%

Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.012 0.068 0.123 0.223 0.43 0.804 1.439 2.532 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.012 0.068 0.123 0.223 0.43 0.804 1.439 2.532 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.012 0.068 0.123 0.223 0.43 0.804 1.439 2.532 O.D. 0.012 0.068 0.123 0.223 0.43 0.804 1.439 2.532

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.