Human Lumican ELISA Kit PicoKine®

Human Lumican ELISA Kit PicoKine® (96 Tests) . Quantitate Human LUM in cell culture supernatants, serum and plasma (heparin) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Lumican, also known as LUM, is a protein which in humans is encoded by the LUM gene. This gene is a member of the small interstitial proteoglycan gene (SIPG) family, and it is a keratan sulfate proteoglycan which presents large quantities in the corneal stroma and in interstitial collagenous matrices of the heart, aorta, skeletal muscle, skin, and intervertebral discs. Lumican is mapped to 12q21.33, it interacts with collagen and limits growth of fibrils in diameter. In the cornea, Lumican not only interacts with collagen molecules to limit fibril growth, but also plays a critical role in the regular spacing of fibrils and acquisition of corneal transparency by virtue of its keratan sulfate-containing glycosaminoglycan side chains LDC.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 349 325 293 350 329 23.19 7% Sample 2 1777 1805 2029 1826 1859 99.53 5.3% Sample 3 5103 4485 4831 4805 4806 219.01 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 349 325 293 350 329 23.19 7% Sample 1 349 325 293 350 329 23.19 7% Sample 2 1777 1805 2029 1826 1859 99.53 5.3% Sample 2 1777 1805 2029 1826 1859 99.53 5.3% Sample 3 5103 4485 4831 4805 4806 219.01 4.5% Sample 3 5103 4485 4831 4805 4806 219.01 4.5%

Synonyms

Lumican; Keratan sulfate proteoglycan lumican; KSPG lumican; LUM; LDC, SLRR2D

Gene Name

LUM

Gene ID

4060

UniProt

P51884

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q19-N338

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Cornea and other tissues.

Applications

ELISA

Field of Research

Cancer, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Invasion/Microenvironment, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 349 1777 5103 367 1619 4802 Standard deviation 23.03 103.06 372.51 24.58 119.8 403.36 CV (%) 6.6% 5.8% 7.3% 6.7% 7.4% 8.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 349 1777 5103 367 1619 4802 Standard deviation 23.03 103.06 372.51 24.58 119.8 403.36 CV (%) 6.6% 5.8% 7.3% 6.7% 7.4% 8.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 349 1777 5103 367 1619 4802 Mean (pg/ml) 349 1777 5103 367 1619 4802 Standard deviation 23.03 103.06 372.51 24.58 119.8 403.36 Standard deviation 23.03 103.06 372.51 24.58 119.8 403.36 CV (%) 6.6% 5.8% 7.3% 6.7% 7.4% 8.4% CV (%) 6.6% 5.8% 7.3% 6.7% 7.4% 8.4%

Sensitivity

<10 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

38429 MW

Specificity

Natural and recombinant human Lumican

Anticoagulant

Heparin

Gene Name Synonym

Lumican

Subcellular Location

Secreted, extracellular space, extracellular matrix .

Sequence Similarities

Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.

Protein Name

Lumican

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.062 0.128 0.206 0.345 0.6256 1.112 1.758 2.274 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.062 0.128 0.206 0.345 0.6256 1.112 1.758 2.274 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.062 0.128 0.206 0.345 0.6256 1.112 1.758 2.274 O.D. 0.062 0.128 0.206 0.345 0.6256 1.112 1.758 2.274

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.