Human FOLR1/Alpha Fr ELISA Kit PicoKine®

Folate receptor 1 adult is a protein that in humans is encoded by the FOLR1 gene. The protein encoded by this gene is a member of the folate receptor (FOLR) family. It is mapped to 11q13.4. FOLR1 expression in Jurkat cells facilitated MBG or EBO entry, and FR-blocking reagents inhibited infection of MBG or EBO. This gene product is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. The FOLR1 gene encodes the adult folate receptor, or folate-binding protein (FBP), which has a high affinity for folic acid and for several reduced folic acid derivatives, and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. FOLR1 is also an important regulator of milk protein synthesis.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 103 108 100 99 102 3.5 3.4% Sample 2 333 359 355 339 346 10.8 3.1% Sample 3 1306 1386 1411 1506 1402 71.35 5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 103 108 100 99 102 3.5 3.4% Sample 1 103 108 100 99 102 3.5 3.4% Sample 2 333 359 355 339 346 10.8 3.1% Sample 2 333 359 355 339 346 10.8 3.1% Sample 3 1306 1386 1411 1506 1402 71.35 5% Sample 3 1306 1386 1411 1506 1402 71.35 5%

Folate receptor alpha; FR-alpha; Adult folate-binding protein; FBP; Folate receptor 1; Folate receptor, adult; KB cells FBP; Ovarian tumor-associated antigen MOv18; FOLR1; FOLR

Primarily expressed in tissues of epithelial origin. Expression is increased in malignant tissues. Expressed in kidney, lung and cerebellum. Detected in placenta and thymus epithelium. .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 103 333 1306 108 312 1388 Standard deviation 7.41 22.31 88.8 8.53 24.96 119.36 CV (%) 7.2% 6.7% 6.8% 7.9% 8% 8.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 103 333 1306 108 312 1388 Standard deviation 7.41 22.31 88.8 8.53 24.96 119.36 CV (%) 7.2% 6.7% 6.8% 7.9% 8% 8.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 103 333 1306 108 312 1388 Mean (pg/ml) 103 333 1306 108 312 1388 Standard deviation 7.41 22.31 88.8 8.53 24.96 119.36 Standard deviation 7.41 22.31 88.8 8.53 24.96 119.36 CV (%) 7.2% 6.7% 6.8% 7.9% 8% 8.6% CV (%) 7.2% 6.7% 6.8% 7.9% 8% 8.6%

Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH. Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release. Required for normal embryonic development and normal cell proliferation. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cell membrane; Lipid-anchor, GPI-anchor. Secreted . Cytoplasmic vesicle. Cytoplasmic vesicle, clathrin-coated vesicle. Endosome. Apical cell membrane . Endocytosed into cytoplasmic vesicles and then recycled to the cell membrane.

Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.032 0.091 0.149 0.259 0.474 0.811 1.385 2.236 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.032 0.091 0.149 0.259 0.474 0.811 1.385 2.236 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.032 0.091 0.149 0.259 0.474 0.811 1.385 2.236 O.D. 0.032 0.091 0.149 0.259 0.474 0.811 1.385 2.236

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.