Human SPINK1/TATI ELISA Kit PicoKine®

Serine protease inhibitor Kazal-type 1, also called TATI or PSTI, is a protein that in humans is encoded by the SPINK1 gene. It is mapped to 5q32. SPINK1 gene contains 4 exons spanning approximately 7.5 kb. It was initially detected in urine of patients with ovarian cancer. This peptide is also produced by the mucosa of the gastrointestinal tract where it may protect the mucosal cells from proteolytic breakdown. The SPINK1 gene encodes pancreatic secretory trypsin inhibitor, which is secreted from pancreatic acinar cells into pancreatic juice. Its physiologic role was thought to be the prevention of trypsin-catalyzed premature activation of zymogens within the pancreas and the pancreatic duct. Mutations in this gene are associated with hereditary pancreatitis and tropical calcific pancreatitis.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 245 238 239 225 236 7.29 3% Sample 2 1862 1592 1831 1832 1779 108.82 6.1% Sample 3 5817 5596 5823 5918 5788 118.14 2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 245 238 239 225 236 7.29 3% Sample 1 245 238 239 225 236 7.29 3% Sample 2 1862 1592 1831 1832 1779 108.82 6.1% Sample 2 1862 1592 1831 1832 1779 108.82 6.1% Sample 3 5817 5596 5823 5918 5788 118.14 2% Sample 3 5817 5596 5823 5918 5788 118.14 2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 245 1862 5817 254 1784 6136 Standard deviation 16.9 137.22 418.82 17.78 148.07 468.33 CV (%) 6.9% 7.3% 7.2% 7% 8.3% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 245 1862 5817 254 1784 6136 Standard deviation 16.9 137.22 418.82 17.78 148.07 468.33 CV (%) 6.9% 7.3% 7.2% 7% 8.3% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 245 1862 5817 254 1784 6136 Mean (pg/ml) 245 1862 5817 254 1784 6136 Standard deviation 16.9 137.22 418.82 17.78 148.07 468.33 Standard deviation 16.9 137.22 418.82 17.78 148.07 468.33 CV (%) 6.9% 7.3% 7.2% 7% 8.3% 7.6% CV (%) 6.9% 7.3% 7.2% 7% 8.3% 7.6%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.07 0.104 0.147 0.223 0.394 0.724 1.237 1.9 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.07 0.104 0.147 0.223 0.394 0.724 1.237 1.9 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.07 0.104 0.147 0.223 0.394 0.724 1.237 1.9 O.D. 0.07 0.104 0.147 0.223 0.394 0.724 1.237 1.9

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.