Human SPINK1/TATI ELISA Kit PicoKine®

Human SPINK1/TATI ELISA Kit PicoKine® (96 Tests) . Quantitate Human SPINK1 in cell culture supernatants, serum and plasma (heparin) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Serine protease inhibitor Kazal-type 1, also called TATI or PSTI, is a protein that in humans is encoded by the SPINK1 gene. It is mapped to 5q32. SPINK1 gene contains 4 exons spanning approximately 7.5 kb. It was initially detected in urine of patients with ovarian cancer. This peptide is also produced by the mucosa of the gastrointestinal tract where it may protect the mucosal cells from proteolytic breakdown. The SPINK1 gene encodes pancreatic secretory trypsin inhibitor, which is secreted from pancreatic acinar cells into pancreatic juice. Its physiologic role was thought to be the prevention of trypsin-catalyzed premature activation of zymogens within the pancreas and the pancreatic duct. Mutations in this gene are associated with hereditary pancreatitis and tropical calcific pancreatitis.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 245 238 239 225 236 7.29 3% Sample 2 1862 1592 1831 1832 1779 108.82 6.1% Sample 3 5817 5596 5823 5918 5788 118.14 2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 245 238 239 225 236 7.29 3% Sample 1 245 238 239 225 236 7.29 3% Sample 2 1862 1592 1831 1832 1779 108.82 6.1% Sample 2 1862 1592 1831 1832 1779 108.82 6.1% Sample 3 5817 5596 5823 5918 5788 118.14 2% Sample 3 5817 5596 5823 5918 5788 118.14 2%

Synonyms

Pancreatic secretory trypsin inhibitor; Serine protease inhibitor Kazal-type 1; Tumor-associated trypsin inhibitor; TATI; SPINK1; PSTI

Gene Name

SPINK1

Gene ID

6690

UniProt

P00995

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: D24-C79

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cell Biology, Protease Inhibitors, Proteolysis/Ubiquitin

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 245 1862 5817 254 1784 6136 Standard deviation 16.9 137.22 418.82 17.78 148.07 468.33 CV (%) 6.9% 7.3% 7.2% 7% 8.3% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 245 1862 5817 254 1784 6136 Standard deviation 16.9 137.22 418.82 17.78 148.07 468.33 CV (%) 6.9% 7.3% 7.2% 7% 8.3% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 245 1862 5817 254 1784 6136 Mean (pg/ml) 245 1862 5817 254 1784 6136 Standard deviation 16.9 137.22 418.82 17.78 148.07 468.33 Standard deviation 16.9 137.22 418.82 17.78 148.07 468.33 CV (%) 6.9% 7.3% 7.2% 7% 8.3% 7.6% CV (%) 6.9% 7.3% 7.2% 7% 8.3% 7.6%

Sensitivity

<10 pg/mL

Function

This is a trypsin inhibitor, its physiological function is to prevent the trypsin-catalyzed premature activation of zymogens within the pancreas.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

8507 MW

Specificity

Natural and recombinant human SPINK1

Anticoagulant

Heparin

Gene Name Synonym

Pancreatic secretory trypsin inhibitor

Subcellular Location

Secreted.

Protein Name

Pancreatic secretory trypsin inhibitor

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.07 0.104 0.147 0.223 0.394 0.724 1.237 1.9 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.07 0.104 0.147 0.223 0.394 0.724 1.237 1.9 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.07 0.104 0.147 0.223 0.394 0.724 1.237 1.9 O.D. 0.07 0.104 0.147 0.223 0.394 0.724 1.237 1.9

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.