Human Midkine ELISA Kit PicoKine®

Midkine (MK or MDK) also known as neurite growth-promoting factor 2 (NEGF2) is a protein that in humans is encoded by the MDK gene. Midkine is a basic heparin-binding growth factor of low molecular weight, and forms a family with pleiotrophin (NEGF1, 46% homologous with MK) . It is a nonglycosylated protein, composed of two domains held by disulfide bridges. It is a developmentally important retinoic acid-responsive gene product strongly induced during mid-gestation, hence the name midkine. Restricted mainly to certain tissues in the normal adult, it is strongly induced during oncogenesis, inflammation and tissue repair. MK is pleiotropic, capable of exerting activities such as cell proliferation, cell migration, angiogenesis and fibrinolysis. A molecular complex containing receptor-type tyrosine phosphatase zeta (PTPζ), low density lipoprotein receptor-related protein (LRP1), anaplastic leukemia kinase (ALK) and syndecans is considered to be its receptor.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 127 130 117 141 128 8.55 6.6% Sample 2 580 634 659 574 611 35.91 5.8% Sample 3 2481 2349 2225 2609 2416 143.56 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 127 130 117 141 128 8.55 6.6% Sample 1 127 130 117 141 128 8.55 6.6% Sample 2 580 634 659 574 611 35.91 5.8% Sample 2 580 634 659 574 611 35.91 5.8% Sample 3 2481 2349 2225 2609 2416 143.56 5.9% Sample 3 2481 2349 2225 2609 2416 143.56 5.9%

Midkine; MK; Amphiregulin-associated protein; ARAP; Midgestation and kidney protein; Neurite outgrowth-promoting factor 2; Neurite outgrowth-promoting protein; MDK; MK1, NEGF2

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 127 580 2481 131 542 2411 Standard deviation 6.22 28.42 155.8 6.68 31.43 184.94 CV (%) 4.9% 4.9% 5.8% 7.7% 5.8% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 127 580 2481 131 542 2411 Standard deviation 6.22 28.42 155.8 6.68 31.43 184.94 CV (%) 4.9% 4.9% 5.8% 7.7% 5.8% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 127 580 2481 131 542 2411 Mean (pg/ml) 127 580 2481 131 542 2411 Standard deviation 6.22 28.42 155.8 6.68 31.43 184.94 Standard deviation 6.22 28.42 155.8 6.68 31.43 184.94 CV (%) 4.9% 4.9% 5.8% 7.7% 5.8% 7.7% CV (%) 4.9% 4.9% 5.8% 7.7% 5.8% 7.7%

Developmentally regulated, secreted growth factor homologous to pleiotrophin (PTN), which has heparin binding activity. Binds anaplastic lymphoma kinase (ALK) which induces ALK activation and subsequent phosphorylation of the insulin receptor substrate (IRS1), followed by the activation of mitogen-activated protein kinase (MAPK) and PI3-kinase, and the induction of cell proliferation. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.035 0.163 0.274 0.458 0.861 1.249 1.832 2.254 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.035 0.163 0.274 0.458 0.861 1.249 1.832 2.254 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.035 0.163 0.274 0.458 0.861 1.249 1.832 2.254 O.D. 0.035 0.163 0.274 0.458 0.861 1.249 1.832 2.254

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.