Human TFF3/ITF ELISA Kit PicoKine®

Human TFF3/Itf ELISA Kit PicoKine® (96 Tests) . Quantitate Human TFF3 in cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Trefoil factor 3 is a protein that in humans is encoded by the TFF3 gene. It is mapped to 21q22.3. Members of the trefoil family can interact with mucins and have an influence on mucus viscosity. They also promote migration of epithelial cells, are linked to antiapoptosis, induce cell scattering, trigger chemotaxis, and participate in immune responses. This gene is a marker of columnar epithelium and is expressed in a variety of tissues including goblet cells of the intestines and colon. It has been found that TFF3 is the most highly upregulated gene in 19 poorly differentiated endometrioid endometrial carcinomas (G3-EECs) compared with normal endometrium biopsies.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 92 86 91 87 89 2.54 2.8% Sample 2 646 631 687 654 654 20.5 3.1% Sample 3 1668 1662 1800 1892 1755 96.19 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 92 86 91 87 89 2.54 2.8% Sample 1 92 86 91 87 89 2.54 2.8% Sample 2 646 631 687 654 654 20.5 3.1% Sample 2 646 631 687 654 654 20.5 3.1% Sample 3 1668 1662 1800 1892 1755 96.19 5.4% Sample 3 1668 1662 1800 1892 1755 96.19 5.4%

Synonyms

Trefoil factor 3; Intestinal trefoil factor; hITF; Polypeptide P1.B; hP1.B; TFF3; ITF, TFI

Gene Name

TFF3

Gene ID

7033

UniProt

Q07654

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: E22-Y80

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from sheep

Tissue Specificity

Expressed in goblet cells of the intestines and colon (at protein level) . Expressed by goblet cells of small and large intestinal epithelia and also by the uterus. Also expressed in the hypothalamus where it is detected in paraventricular, periventricular and supraoptic nuclei (at protein level) . .

Applications

ELISA

Field of Research

Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine.

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 92 646 1668 89 679 1553 Standard deviation 5.06 41.34 78.39 6.4%9 51.6 100.94 CV (%) 5.5% 6.4% 4.7% 6.4% 7.6% 6.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 92 646 1668 89 679 1553 Standard deviation 5.06 41.34 78.39 6.4%9 51.6 100.94 CV (%) 5.5% 6.4% 4.7% 6.4% 7.6% 6.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 92 646 1668 89 679 1553 Mean (pg/ml) 92 646 1668 89 679 1553 Standard deviation 5.06 41.34 78.39 6.4%9 51.6 100.94 Standard deviation 5.06 41.34 78.39 6.4%9 51.6 100.94 CV (%) 5.5% 6.4% 4.7% 6.4% 7.6% 6.5% CV (%) 5.5% 6.4% 4.7% 6.4% 7.6% 6.5%

Sensitivity

<10 pg/mL

Function

Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen) . .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

8641 MW

Specificity

Natural and recombinant human TFF3

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Trefoil factor 3

Subcellular Location

Secreted, extracellular space, extracellular matrix . Cytoplasm .

Sequence Similarities

Contains 1 P-type (trefoil) domain.

Protein Name

Trefoil factor 3

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.03 0.115 0.21 0.345 0.557 0.9 1.384 1.909 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.03 0.115 0.21 0.345 0.557 0.9 1.384 1.909 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.03 0.115 0.21 0.345 0.557 0.9 1.384 1.909 O.D. 0.03 0.115 0.21 0.345 0.557 0.9 1.384 1.909

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.