Human TFF3/ITF ELISA Kit PicoKine®

Trefoil factor 3 is a protein that in humans is encoded by the TFF3 gene. It is mapped to 21q22.3. Members of the trefoil family can interact with mucins and have an influence on mucus viscosity. They also promote migration of epithelial cells, are linked to antiapoptosis, induce cell scattering, trigger chemotaxis, and participate in immune responses. This gene is a marker of columnar epithelium and is expressed in a variety of tissues including goblet cells of the intestines and colon. It has been found that TFF3 is the most highly upregulated gene in 19 poorly differentiated endometrioid endometrial carcinomas (G3-EECs) compared with normal endometrium biopsies.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 92 86 91 87 89 2.54 2.8% Sample 2 646 631 687 654 654 20.5 3.1% Sample 3 1668 1662 1800 1892 1755 96.19 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 92 86 91 87 89 2.54 2.8% Sample 1 92 86 91 87 89 2.54 2.8% Sample 2 646 631 687 654 654 20.5 3.1% Sample 2 646 631 687 654 654 20.5 3.1% Sample 3 1668 1662 1800 1892 1755 96.19 5.4% Sample 3 1668 1662 1800 1892 1755 96.19 5.4%

Expressed in goblet cells of the intestines and colon (at protein level) . Expressed by goblet cells of small and large intestinal epithelia and also by the uterus. Also expressed in the hypothalamus where it is detected in paraventricular, periventricular and supraoptic nuclei (at protein level) . .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 92 646 1668 89 679 1553 Standard deviation 5.06 41.34 78.39 6.4%9 51.6 100.94 CV (%) 5.5% 6.4% 4.7% 6.4% 7.6% 6.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 92 646 1668 89 679 1553 Standard deviation 5.06 41.34 78.39 6.4%9 51.6 100.94 CV (%) 5.5% 6.4% 4.7% 6.4% 7.6% 6.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 92 646 1668 89 679 1553 Mean (pg/ml) 92 646 1668 89 679 1553 Standard deviation 5.06 41.34 78.39 6.4%9 51.6 100.94 Standard deviation 5.06 41.34 78.39 6.4%9 51.6 100.94 CV (%) 5.5% 6.4% 4.7% 6.4% 7.6% 6.5% CV (%) 5.5% 6.4% 4.7% 6.4% 7.6% 6.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.03 0.115 0.21 0.345 0.557 0.9 1.384 1.909 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.03 0.115 0.21 0.345 0.557 0.9 1.384 1.909 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.03 0.115 0.21 0.345 0.557 0.9 1.384 1.909 O.D. 0.03 0.115 0.21 0.345 0.557 0.9 1.384 1.909

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.