Mouse Relaxin 1 ELISA Kit PicoKine®

Mouse Relaxin 1 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Rln1 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Relaxin 1 is a member of relaxin-like peptide family. Relaxin gene maps to mouse chromosome 19 near D19Mit23. Relaxin is a peptide hormone produced by the corpora lutea of ovaries during pregnancy in many mammalian species, including man. Relaxin widens the pubic bone and facilitates labor, it also softens the cervix (cervical ripening), and relaxes the uterine musculature. However, its significance may reach much further. Relaxin affects collagen metabolism, inhibiting collagen synthesis and enhancing its breakdown by increasing matrix metalloproteinases. It also enhances angiogenesis and is a potent renal vasodilator.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 104 106 113 116 109 4.91 4.5% Sample 2 612 581 605 608 601 12.09 2% Sample 3 2407 2396 2116 2524 2360 149.94 6.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 104 106 113 116 109 4.91 4.5% Sample 1 104 106 113 116 109 4.91 4.5% Sample 2 612 581 605 608 601 12.09 2% Sample 2 612 581 605 608 601 12.09 2% Sample 3 2407 2396 2116 2524 2360 149.94 6.3% Sample 3 2407 2396 2116 2524 2360 149.94 6.3%

Synonyms

Prorelaxin 1; Relaxin B chain; Relaxin A chain; Rln1; Rln, Rlx

Gene Name

RLN1

Gene ID

19773

UniProt

P47932

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: R23-R54 (B) & E161-C185 (A)

Capture Antibody

Polyclonal from sheep

Detection Antibody

Polyclonal from sheep

Applications

ELISA

Field of Research

Cell Type Markers, Growth Factors/Hormones, Signal Transduction, Tags & Cell Markers

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

78 pg/mL - 5,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 104 612 2407 108 616 2227 Standard deviation 4.16 47.12 154.04 7.71 59.13 144.75 CV (%) 4% 7.7% 6.4% 5.2% 9.6% 6.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 104 612 2407 108 616 2227 Standard deviation 4.16 47.12 154.04 7.71 59.13 144.75 CV (%) 4% 7.7% 6.4% 5.2% 9.6% 6.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 104 612 2407 108 616 2227 Mean (pg/ml) 104 612 2407 108 616 2227 Standard deviation 4.16 47.12 154.04 7.71 59.13 144.75 Standard deviation 4.16 47.12 154.04 7.71 59.13 144.75 CV (%) 4% 7.7% 6.4% 5.2% 9.6% 6.5% CV (%) 4% 7.7% 6.4% 5.2% 9.6% 6.5%

Sensitivity

<10 pg/mL

Function

Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

20571 MW

Specificity

Natural and recombinant mouse Relaxin 1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Prorelaxin 1

Subcellular Location

Secreted.

Protein Name

Prorelaxin 1

Sample Data

Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.047 0.164 0.276 0.445 0.698 1.136 1.619 1.913 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.047 0.164 0.276 0.445 0.698 1.136 1.619 1.913 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.047 0.164 0.276 0.445 0.698 1.136 1.619 1.913 O.D. 0.047 0.164 0.276 0.445 0.698 1.136 1.619 1.913

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.