Mouse JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine®

Junctional adhesion molecule A (JAM-A) is a protein that in humans is encoded by the F11R gene. It is mapped to 1q23.3. This gene is an immunoglobulin-like molecule that colocalizes with tight junctions in endothelium and epithelium and is also found on blood leukocytes and platelets. JAM-A plays an important role in the regulation of tight junction assembly in epithelia. In addition, it can act as a receptor for reovirus, a ligand for the integrin LFA1, involved in leukocyte transmigration, and a platelet receptor. JAM-A has a nonredundant role in controlling DC motility, trafficking to lymph nodes, and activation of specific immunity.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 74 76 83 81 78 3.64 4.6% Sample 2 243 243 259 220 241 13.89 5.7% Sample 3 871 854 977 972 918 56.34 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 74 76 83 81 78 3.64 4.6% Sample 1 74 76 83 81 78 3.64 4.6% Sample 2 243 243 259 220 241 13.89 5.7% Sample 2 243 243 259 220 241 13.89 5.7% Sample 3 871 854 977 972 918 56.34 6.1% Sample 3 871 854 977 972 918 56.34 6.1%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 74 243 871 67 225 792 Standard deviation 3.18 18.95 57.48 3.28 18.67 61.77 CV (%) 4.3% 7.8% 6.6% 4.9% 8.3% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 74 243 871 67 225 792 Standard deviation 3.18 18.95 57.48 3.28 18.67 61.77 CV (%) 4.3% 7.8% 6.6% 4.9% 8.3% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 74 243 871 67 225 792 Mean (pg/ml) 74 243 871 67 225 792 Standard deviation 3.18 18.95 57.48 3.28 18.67 61.77 Standard deviation 3.18 18.95 57.48 3.28 18.67 61.77 CV (%) 4.3% 7.8% 6.6% 4.9% 8.3% 7.8% CV (%) 4.3% 7.8% 6.6% 4.9% 8.3% 7.8%

Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly. Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cell junction, tight junction . Cell membrane ; Single-pass type I membrane protein . Localized at tight junctions of both epithelial and endothelial cells.

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.075 0.265 0.352 0.524 0.786 1.196 1.802 2.603 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.075 0.265 0.352 0.524 0.786 1.196 1.802 2.603 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.075 0.265 0.352 0.524 0.786 1.196 1.802 2.603 O.D. 0.075 0.265 0.352 0.524 0.786 1.196 1.802 2.603

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.