Mouse JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine®

Mouse JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse F11r in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Junctional adhesion molecule A (JAM-A) is a protein that in humans is encoded by the F11R gene. It is mapped to 1q23.3. This gene is an immunoglobulin-like molecule that colocalizes with tight junctions in endothelium and epithelium and is also found on blood leukocytes and platelets. JAM-A plays an important role in the regulation of tight junction assembly in epithelia. In addition, it can act as a receptor for reovirus, a ligand for the integrin LFA1, involved in leukocyte transmigration, and a platelet receptor. JAM-A has a nonredundant role in controlling DC motility, trafficking to lymph nodes, and activation of specific immunity.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 74 76 83 81 78 3.64 4.6% Sample 2 243 243 259 220 241 13.89 5.7% Sample 3 871 854 977 972 918 56.34 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 74 76 83 81 78 3.64 4.6% Sample 1 74 76 83 81 78 3.64 4.6% Sample 2 243 243 259 220 241 13.89 5.7% Sample 2 243 243 259 220 241 13.89 5.7% Sample 3 871 854 977 972 918 56.34 6.1% Sample 3 871 854 977 972 918 56.34 6.1%

Synonyms

Junctional adhesion molecule A; JAM-A; Junctional adhesion molecule 1; JAM-1; CD321; F11r; Jam1, Jcam, Jcam1

Gene Name

F11R

Gene ID

16456

UniProt

O88792

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: K27-G238

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Atherosclerosis, Cardiovascular, Cell Adhesion, Cytoskeleton/ECM, Platelets, Signal Transduction, Tight Junctions, Vascular Inflammation

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 74 243 871 67 225 792 Standard deviation 3.18 18.95 57.48 3.28 18.67 61.77 CV (%) 4.3% 7.8% 6.6% 4.9% 8.3% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 74 243 871 67 225 792 Standard deviation 3.18 18.95 57.48 3.28 18.67 61.77 CV (%) 4.3% 7.8% 6.6% 4.9% 8.3% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 74 243 871 67 225 792 Mean (pg/ml) 74 243 871 67 225 792 Standard deviation 3.18 18.95 57.48 3.28 18.67 61.77 Standard deviation 3.18 18.95 57.48 3.28 18.67 61.77 CV (%) 4.3% 7.8% 6.6% 4.9% 8.3% 7.8% CV (%) 4.3% 7.8% 6.6% 4.9% 8.3% 7.8%

Sensitivity

<10 pg/mL

Function

Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly. Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus (By similarity) . .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

32424 MW

Specificity

Natural and recombinant mouse JAM-A

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Junctional adhesion molecule A

Subcellular Location

Cell junction, tight junction . Cell membrane ; Single-pass type I membrane protein . Localized at tight junctions of both epithelial and endothelial cells.

Sequence Similarities

Belongs to the immunoglobulin superfamily.

Protein Name

Junctional adhesion molecule A

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.075 0.265 0.352 0.524 0.786 1.196 1.802 2.603 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.075 0.265 0.352 0.524 0.786 1.196 1.802 2.603 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.075 0.265 0.352 0.524 0.786 1.196 1.802 2.603 O.D. 0.075 0.265 0.352 0.524 0.786 1.196 1.802 2.603

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.