Mouse TNFRSF14/HVEM ELISA Kit PicoKine®

Tumor necrosis factor receptor superfamily member 14 (TNFRSF14), also known as HVEM, is a protein that in humans is encoded by the TNFRSF14 gene. The protein encoded by this gene is a member of the TNF-receptor superfamily. It is mapped to 1p36.32. HVEM plays an important role in HSV pathogenesis because it enhanced the entry of several wildtype HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T cells. HVEM and BTLA which are form a biional signaling pathway can regulate cell survival and inhibitory responses between interacting cells. HVEM as an important orchestrator of mucosal immunity integrates signals from innate lymphocytes to induce optimal epithelial Stat3 activation, which indicated that targeting HVEM with agonists could improve host defense.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 29 31 29 29 29 0.86 2.9% Sample 2 218 201 191 189 199 11.47 5.7% Sample 3 439 461 413 382 423 29.49 6.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 29 31 29 29 29 0.86 2.9% Sample 1 29 31 29 29 29 0.86 2.9% Sample 2 218 201 191 189 199 11.47 5.7% Sample 2 218 201 191 189 199 11.47 5.7% Sample 3 439 461 413 382 423 29.49 6.9% Sample 3 439 461 413 382 423 29.49 6.9%

Antiviral Signaling, Cancer, Cytokines, Growth Factors, Growth Factors/Hormones, Immune System Diseases, Immunology, Innate Immunity, Signal Transduction, TNF Superfamily

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 29 218 439 28 197 475 Standard deviation 1.59 14.38 26.77 1.56 16.15 31.82 CV (%) 5.5% 6.6% 6.1% 5.6% 8.2% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 29 218 439 28 197 475 Standard deviation 1.59 14.38 26.77 1.56 16.15 31.82 CV (%) 5.5% 6.6% 6.1% 5.6% 8.2% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 29 218 439 28 197 475 Mean (pg/ml) 29 218 439 28 197 475 Standard deviation 1.59 14.38 26.77 1.56 16.15 31.82 Standard deviation 1.59 14.38 26.77 1.56 16.15 31.82 CV (%) 5.5% 6.6% 6.1% 5.6% 8.2% 6.7% CV (%) 5.5% 6.6% 6.1% 5.6% 8.2% 6.7%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.022 0.053 0.12 0.254 0.454 0.779 1.301 1.985 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.022 0.053 0.12 0.254 0.454 0.779 1.301 1.985 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.022 0.053 0.12 0.254 0.454 0.779 1.301 1.985 O.D. 0.022 0.053 0.12 0.254 0.454 0.779 1.301 1.985

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.