Mouse CD56 / NCAM-1 / NCAM1 ELISA Kit PicoKine®

Mouse CD56 / NCAM-1 / NCAM1 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Ncam1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Neural Cell Adhesion Molecule, NCAM, also known as the cluster of differentiation CD56, is a hemophilic binding glycoprotein. It is a glycoprotein of Immunoglobulin (Ig) super family. At least 27 alternatively spliced NCAM mRNAs are produced, giving a wide diversity of NCAM isoforms NCAM gene is located at 11q22-q23.This glycoprotein is mainly expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cell–cell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1344 1609 1386 1481 1455 101.82 6.9% Sample 2 4632 4852 4530 4967 4745 173 3.6% Sample 3 15378 17338 16502 16161 16344 703.49 4.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1344 1609 1386 1481 1455 101.82 6.9% Sample 1 1344 1609 1386 1481 1455 101.82 6.9% Sample 2 4632 4852 4530 4967 4745 173 3.6% Sample 2 4632 4852 4530 4967 4745 173 3.6% Sample 3 15378 17338 16502 16161 16344 703.49 4.3% Sample 3 15378 17338 16502 16161 16344 703.49 4.3%

Synonyms

Neural cell adhesion molecule 1; N-CAM-1; NCAM-1; CD56; Ncam1; Ncam

Gene Name

NCAM1

Gene ID

17967

UniProt

P13595

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L20-T711

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Axonal Guidance Proteins, Cell Adhesion Proteins, Cell Type Marker, Developmental Biology, ECM Proteins, Growth and Development, Hematopoietic Progenitors, Membrane Proteins, Nervous System Development, Neural Signal Transduction, Neurology Process, Neuron Marker, Neuroscience, Organogenesis, Stem Cells

Standard

40 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

625 pg/mL - 40,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1344 4632 15378 1401 4645 16195 Standard deviation 90.04 342.76 953.43 120.48 422.69 1214.62 CV (%) 6.7% 7.4% 6.2% 8.6% 9.1% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1344 4632 15378 1401 4645 16195 Standard deviation 90.04 342.76 953.43 120.48 422.69 1214.62 CV (%) 6.7% 7.4% 6.2% 8.6% 9.1% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1344 4632 15378 1401 4645 16195 Mean (pg/ml) 1344 4632 15378 1401 4645 16195 Standard deviation 90.04 342.76 953.43 120.48 422.69 1214.62 Standard deviation 90.04 342.76 953.43 120.48 422.69 1214.62 CV (%) 6.7% 7.4% 6.2% 8.6% 9.1% 7.5% CV (%) 6.7% 7.4% 6.2% 8.6% 9.1% 7.5%

Sensitivity

<20 pg/mL

Function

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

119427 MW

Specificity

Natural and recombinant mouse CD56

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Neural cell adhesion molecule 1

Subcellular Location

Isoform 1: Cell membrane; Single-pass type I membrane protein.

Sequence Similarities

Contains 2 fibronectin type-III domains.

Protein Name

Neural cell adhesion molecule 1

Sample Data

Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.038 0.143 0.268 0.411 0.772 1.3 1.634 1.944 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.038 0.143 0.268 0.411 0.772 1.3 1.634 1.944 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.038 0.143 0.268 0.411 0.772 1.3 1.634 1.944 O.D. 0.038 0.143 0.268 0.411 0.772 1.3 1.634 1.944

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.