Mouse CD13/Aminopeptidase N ELISA Kit PicoKine®

Alanine aminopeptidase, also known as ANPEP or CD13. is an enzyme that is used as a biomarker to detect damage to the kidneys, and that may be used to help diagnose certain kidney disorders. It is mapped to 15q26.1. Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine, Aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases, and it is also thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Petrovic et al showed that CD13 was required for endothelial cell invasion in response to bradykinin. Inhibition of CD13 abrogated internalization of bradykinin receptor B2 and reduced endothelial cell motility.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 642 646 652 682 655 15.7 2.3% Sample 2 1721 1522 1546 1514 1575 84.68 5.3% Sample 3 9368 9521 9150 9445 9371 138.58 1.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 642 646 652 682 655 15.7 2.3% Sample 1 642 646 652 682 655 15.7 2.3% Sample 2 1721 1522 1546 1514 1575 84.68 5.3% Sample 2 1721 1522 1546 1514 1575 84.68 5.3% Sample 3 9368 9521 9150 9445 9371 138.58 1.4% Sample 3 9368 9521 9150 9445 9371 138.58 1.4%

Aminopeptidase N; AP-N; mAPN;3.4.11.2; Alanyl aminopeptidase; Aminopeptidase M; AP-M; Membrane protein p161; Microsomal aminopeptidase; CD13; Anpep; Lap-1, Lap1

Highly expressed in intestinal tract and kidney, present in liver, lymph node, spleen, and brain. Found as well in monocytes, macrophages, dendritic cells, veiled cells and B-cells but not on T-cells and thymocytes. .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 642 1721 9368 660 1550 9748 Standard deviation 50.07 134.23 543.34 57.42 127.1 633.62 CV (%) 7.8% 7.8% 5.8% 8.7% 8.2% 6.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 642 1721 9368 660 1550 9748 Standard deviation 50.07 134.23 543.34 57.42 127.1 633.62 CV (%) 7.8% 7.8% 5.8% 8.7% 8.2% 6.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 642 1721 9368 660 1550 9748 Mean (pg/ml) 642 1721 9368 660 1550 9748 Standard deviation 50.07 134.23 543.34 57.42 127.1 633.62 Standard deviation 50.07 134.23 543.34 57.42 127.1 633.62 CV (%) 7.8% 7.8% 5.8% 8.7% 8.2% 6.5% CV (%) 7.8% 7.8% 5.8% 8.7% 8.2% 6.5%

Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May be involved in the metabolism of regulatory peptides of diverse cell types, responsible for the processing of peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. May have a role in angiogenesis (By similarity) . Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.039 0.173 0.281 0.472 0.784 1.236 1.732 2.01 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.039 0.173 0.281 0.472 0.784 1.236 1.732 2.01 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.039 0.173 0.281 0.472 0.784 1.236 1.732 2.01 O.D. 0.039 0.173 0.281 0.472 0.784 1.236 1.732 2.01

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.