Human soluble CD5 ELISA Kit PicoKine®

Human soluble CD5 ELISA Kit PicoKine® (96 Tests) . Quantitate Human CD5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CD5, also known as T1 or LEU1, is a cluster of differentiation found on a subset of IgM-secreting B cells called B-1 cells, and also on T cells. It is mapped to 11q12.2. Although CD5 can be found on both T cells and B cells, T cells express higher levels of CD5 than B cells. CD5 was used as a T-cell marker until monoclonal antibodies against CD3 were developed. CD5 is upregulated on T cells upon strong activation. In the thymus, there is a correlation with CD5 expression and strength of the interaction of the T cell towards self-peptides. Nevertheless, CD5 serves to mitigate activating signals from the BCR so that the B-1 cells can only be activated by very strong stimuli (such as bacterial proteins) and not by normal tissue proteins.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 30 29 32 28 29 1.47 5% Sample 2 156 166 150 158 157 5.72 3.6% Sample 3 497 448 471 476 473 17.42 3.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 30 29 32 28 29 1.47 5% Sample 1 30 29 32 28 29 1.47 5% Sample 2 156 166 150 158 157 5.72 3.6% Sample 2 156 166 150 158 157 5.72 3.6% Sample 3 497 448 471 476 473 17.42 3.6% Sample 3 497 448 471 476 473 17.42 3.6%

Synonyms

T-cell surface glycoprotein CD5; Lymphocyte antigen T1/Leu-1; CD5; CD5; LEU1

Gene Name

CD5

Gene ID

921

UniProt

P06127

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: R25-N371

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Adaptive Immunity, B Cells, Hematopoietic Progenitors, Immunology, Lymphoid, Stem Cells, T Cells, T Lymphocytic Lineage

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 32 263 425 33 253 425 Standard deviation 1.69 11.04 28.05 2.27 17.28 30.6 CV (%) 5.3% 4.2% 6.6% 6.9% 6% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 32 263 425 33 253 425 Standard deviation 1.69 11.04 28.05 2.27 17.28 30.6 CV (%) 5.3% 4.2% 6.6% 6.9% 6% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 32 263 425 33 253 425 Mean (pg/ml) 32 263 425 33 253 425 Standard deviation 1.69 11.04 28.05 2.27 17.28 30.6 Standard deviation 1.69 11.04 28.05 2.27 17.28 30.6 CV (%) 5.3% 4.2% 6.6% 6.9% 6% 7.2% CV (%) 5.3% 4.2% 6.6% 6.9% 6% 7.2%

Sensitivity

<10 pg/mL

Function

May act as a receptor in regulating T-cell proliferation.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

54578 MW

Specificity

Natural and recombinant human CD5

Anticoagulant

Heparin or EDTA

Gene Name Synonym

T-cell surface glycoprotein CD5

Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Protein Name

T-cell surface glycoprotein CD5

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.044 0.101 0.136 0.217 0.404 0.747 1.384 2.021 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.044 0.101 0.136 0.217 0.404 0.747 1.384 2.021 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.044 0.101 0.136 0.217 0.404 0.747 1.384 2.021 O.D. 0.044 0.101 0.136 0.217 0.404 0.747 1.384 2.021

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.