Mouse uPAR / uPA Receptor ELISA Kit PicoKine®

The urokinase-type plasminogen activator receptor (uPAR) is a key molecule in the regulation of cell-surface plasminogen activation and, as such, plays an important role in many normal as well as pathological processes.1 The cDNA for Mo3, an activation antigen expressed by human monocytes and myelomonocytic cell lines after stimulation by a variety of agents. Mo3 expression in vivo is associated predominantly with macrophages in inflammatory sites. It is a highly glycosylated protein of about 50 kD in monocytes where it is anchored to the plasma membrane by glycosyl-phosphatidylinositol linkage. The complete coding sequence of the cDNA has been found to encode 335 amino acids including a predicted signal peptide of 22 residues and a hydrophobic C-terminal portion. Mo3 is identical to the human receptor for the urokinase plasminogen activator.2 UPAR is a useful prognostic marker for biologically aggressive forms of endometrial cancer.3 PLAUR is located at chromosome 19q13.1-q13.2.1

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 102 93 88 95 94 5.02 5.3% Sample 2 569 530 548 568 553 16.06 2.9% Sample 3 1345 1270 1379 1260 1313 50.09 3.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 102 93 88 95 94 5.02 5.3% Sample 1 102 93 88 95 94 5.02 5.3% Sample 2 569 530 548 568 553 16.06 2.9% Sample 2 569 530 548 568 553 16.06 2.9% Sample 3 1345 1270 1379 1260 1313 50.09 3.8% Sample 3 1345 1270 1379 1260 1313 50.09 3.8%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 102 569 1345 111 532 1266 Standard deviation 7.95 40.39 71.28 10.21 42.02 81.02 CV (%) 7.8% 7.1% 5.3% 9.2% 7.9% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 102 569 1345 111 532 1266 Standard deviation 7.95 40.39 71.28 10.21 42.02 81.02 CV (%) 7.8% 7.1% 5.3% 9.2% 7.9% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 102 569 1345 111 532 1266 Mean (pg/ml) 102 569 1345 111 532 1266 Standard deviation 7.95 40.39 71.28 10.21 42.02 81.02 Standard deviation 7.95 40.39 71.28 10.21 42.02 81.02 CV (%) 7.8% 7.1% 5.3% 9.2% 7.9% 6.4% CV (%) 7.8% 7.1% 5.3% 9.2% 7.9% 6.4%

Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.006 0.082 0.186 0.378 0.759 1.218 1.77 1.956 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.006 0.082 0.186 0.378 0.759 1.218 1.77 1.956 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.006 0.082 0.186 0.378 0.759 1.218 1.77 1.956 O.D. 0.006 0.082 0.186 0.378 0.759 1.218 1.77 1.956

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.