Human SPARC ELISA Kit PicoKine®

SPARC (secreted protein acidic and rich in cysteine), also known as Osteonectin, is a protein that in humans is encoded by the SPARC gene. The human SPARC gene is 26.5 kb long, and contains 10 exons and 9 introns and is located on chromosome 5q31-q33. SPARC is a glycoprotein of 40 kD. SPARC is an acidic, cysteine-rich glycoprotein consisting of a single polypeptide chain that can be broken into 4 domains: 1) an Ca++ binding domains near the glutamic acidic-rich region at the amino terminus (domain I), 2) a cysteine- rich (domain II), 3) a hydrophilic region (domain III) and 4) an EF hand motif at the carboxy terminus region (domain IV) . Osteonectin is a glycoprotein in the bone that binds sodium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. Osteonectin also shows affinity for collagen in addition to bone mineral calcium. A correlation between osteonectin over expression and ampullary cancers and chronic pancreatitis has been found.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1247 1275 1224 1339 1271 43.08 3.3% Sample 2 5311 5517 5748 5864 5610 213.07 3.7% Sample 3 21553 20541 21138 20396 20907 465.22 2.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1247 1275 1224 1339 1271 43.08 3.3% Sample 1 1247 1275 1224 1339 1271 43.08 3.3% Sample 2 5311 5517 5748 5864 5610 213.07 3.7% Sample 2 5311 5517 5748 5864 5610 213.07 3.7% Sample 3 21553 20541 21138 20396 20907 465.22 2.2% Sample 3 21553 20541 21138 20396 20907 465.22 2.2%

Angiogenesis, Cancer, Cardiovascular, Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Cytoskeleton/ECM, Developmental Biology, Extracellular Matrix, Lineage Markers, Lineage Specification, Mesenchymal Stem Cells, Protein Phosphorylation, Receptor Tyrosine Kinases, Signal Transduction, Stem Cells, Structures, Tyrosine Kinases

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1247 5311 21553 1248 5203 22753 Standard deviation 93.52 393.01 1012.99 99.84 457.86 1296.92 CV (%) 7.5% 7.4% 4.7% 8% 8.8% 5.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1247 5311 21553 1248 5203 22753 Standard deviation 93.52 393.01 1012.99 99.84 457.86 1296.92 CV (%) 7.5% 7.4% 4.7% 8% 8.8% 5.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1247 5311 21553 1248 5203 22753 Mean (pg/ml) 1247 5311 21553 1248 5203 22753 Standard deviation 93.52 393.01 1012.99 99.84 457.86 1296.92 Standard deviation 93.52 393.01 1012.99 99.84 457.86 1296.92 CV (%) 7.5% 7.4% 4.7% 8% 8.8% 5.7% CV (%) 7.5% 7.4% 4.7% 8% 8.8% 5.7%

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca (2+) with a low affinity and an EF-hand loop that binds a Ca (2+) ion with a high affinity.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.061 0.131 0.208 0.345 0.579 0.936 1.478 2.187 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.061 0.131 0.208 0.345 0.579 0.936 1.478 2.187 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.061 0.131 0.208 0.345 0.579 0.936 1.478 2.187 O.D. 0.061 0.131 0.208 0.345 0.579 0.936 1.478 2.187

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.