Rat SAP/PTX2/APCS ELISA Kit PicoKine®

Amyloid P component, serum (SAP), also known as PTX2 or APCS, is the identical serum form of amyloid P component (AP), a 25kDa pentameric protein first identified as the pentagonal constituent of in vivo pathological deposits called amyloid. It belongs to the pentraxins family, characterised by calcium dependent ligand binding and distinctive flattened beta-jellyroll structure similar to that of the legume lectins. This gene is mapped to 1q23.2. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 273 296 296 294 289 9.70 3.3% Sample 2 768 781 870 836 813 41.30 5.0% Sample 3 3986 3724 3655 3712 3769 127.82 3.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 273 296 296 294 289 9.70 3.3% Sample 1 273 296 296 294 289 9.70 3.3% Sample 2 768 781 870 836 813 41.30 5.0% Sample 2 768 781 870 836 813 41.30 5.0% Sample 3 3986 3724 3655 3712 3769 127.82 3.3% Sample 3 3986 3724 3655 3712 3769 127.82 3.3%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 289 813 3769 294 1163 4012 Standard deviation 13.87 46.34 173.37 14.99 68.62 256.77 CV (%) 4.8% 5.7% 4.6% 5.1% 5.9% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 289 813 3769 294 1163 4012 Standard deviation 13.87 46.34 173.37 14.99 68.62 256.77 CV (%) 4.8% 5.7% 4.6% 5.1% 5.9% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 289 813 3769 294 1163 4012 Mean (pg/ml) 289 813 3769 294 1163 4012 Standard deviation 13.87 46.34 173.37 14.99 68.62 256.77 Standard deviation 13.87 46.34 173.37 14.99 68.62 256.77 CV (%) 4.8% 5.7% 4.6% 5.1% 5.9% 6.4% CV (%) 4.8% 5.7% 4.6% 5.1% 5.9% 6.4%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.077 0.138 0.192 0.292 0.504 0.835 1.404 1.897 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.077 0.138 0.192 0.292 0.504 0.835 1.404 1.897 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.077 0.138 0.192 0.292 0.504 0.835 1.404 1.897 O.D. 0.077 0.138 0.192 0.292 0.504 0.835 1.404 1.897

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.