Human PSP94/MSMB ELISA Kit PicoKine®

Beta-microseminoprotein, also known as MSMB or PSP94, is a protein that in humans is encoded by the MSMB gene. It is a member of the immunoglobulin binding factor family. This gene is mapped to 10q11.23. It is synthesized by the epithelial cells of the prostate gland and secreted into the seminal plasma. The expression of the encoded protein is found to be decreased in prostate cancer. But transcripts of identical size were found in prostate, respiratory (tracheal, bronchial, and lung) tissues, and the mucosa of the gastric antrum. Thus, the protein may have functions other than those related to male reproduction. This protein has inhibin-like activity. It may have a role as an autocrine paracrine factor in uterine, breast and other female reproductive tissues.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 160 156 160 151 156 3.69 2.3% Sample 2 628 662 671 720 670 32.89 4.9% Sample 3 1438 1274 1320 1318 1337 60.86 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 160 156 160 151 156 3.69 2.3% Sample 1 160 156 160 151 156 3.69 2.3% Sample 2 628 662 671 720 670 32.89 4.9% Sample 2 628 662 671 720 670 32.89 4.9% Sample 3 1438 1274 1320 1318 1337 60.86 4.5% Sample 3 1438 1274 1320 1318 1337 60.86 4.5%

Beta-microseminoprotein; Immunoglobulin-binding factor; IGBF; PN44; Prostate secreted seminal plasma protein; Prostate secretory protein of 94 amino acids; PSP-94; PSP94; Seminal plasma beta-inhibin; MSMB; PRSP

Strongly expressed in prostate, liver, kidney, breast and penis. Also expressed in pancreas, esophagus, stomach, deodenum, colon, trachea, lung, salivary glands and fallopian tube. PSP94 is expressed in lung and breast, whereas PSP57 is found in kidney and bladder. .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 160 628 1438 148 589 1345 Standard deviation 9.44 49.61 113.6 8.73 53.59 130.46 CV (%) 7.9% 7.9% 7.9% 5.9% 9.1% 9.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 160 628 1438 148 589 1345 Standard deviation 9.44 49.61 113.6 8.73 53.59 130.46 CV (%) 7.9% 7.9% 7.9% 5.9% 9.1% 9.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 160 628 1438 148 589 1345 Mean (pg/ml) 160 628 1438 148 589 1345 Standard deviation 9.44 49.61 113.6 8.73 53.59 130.46 Standard deviation 9.44 49.61 113.6 8.73 53.59 130.46 CV (%) 7.9% 7.9% 7.9% 5.9% 9.1% 9.7% CV (%) 7.9% 7.9% 7.9% 5.9% 9.1% 9.7%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.004 0.057 0.111 0.207 0.371 0.733 1.373 2.009 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.004 0.057 0.111 0.207 0.371 0.733 1.373 2.009 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.004 0.057 0.111 0.207 0.371 0.733 1.373 2.009 O.D. 0.004 0.057 0.111 0.207 0.371 0.733 1.373 2.009

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.