Mouse TSLP ELISA Kit PicoKine®

Thymic stromal lymphopoietin, also called TSLP, is a protein belonging to the cytokine family. This gene is mapped to 5q22.1. It encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c (+) dendritic cells. The protein promotes T helper type 2 (TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, allergic inflammation and chronic obstructive pulmonary disease. The protein is therefore considered a potential therapeutic target for the treatment of such diseases.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 32 33 31 32 1.11 3.4% Sample 2 252 250 232 230 241 10.04 4.1% Sample 3 469 440 476 463 462 13.5 2.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 32 33 31 32 1.11 3.4% Sample 1 34 32 33 31 32 1.11 3.4% Sample 2 252 250 232 230 241 10.04 4.1% Sample 2 252 250 232 230 241 10.04 4.1% Sample 3 469 440 476 463 462 13.5 2.9% Sample 3 469 440 476 463 462 13.5 2.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 252 469 33 246 427 Standard deviation 1.8 15.12 36.58 1.74 15.25 38.43 CV (%) 5.3% 6% 7.8% 5.3% 6.2% 9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 252 469 33 246 427 Standard deviation 1.8 15.12 36.58 1.74 15.25 38.43 CV (%) 5.3% 6% 7.8% 5.3% 6.2% 9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 34 252 469 33 246 427 Mean (pg/ml) 34 252 469 33 246 427 Standard deviation 1.8 15.12 36.58 1.74 15.25 38.43 Standard deviation 1.8 15.12 36.58 1.74 15.25 38.43 CV (%) 5.3% 6% 7.8% 5.3% 6.2% 9% CV (%) 5.3% 6% 7.8% 5.3% 6.2% 9%

Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c (+) dendritic cells. Can induce allergic inflammation by ly activating mast cells (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.066 0.098 0.164 0.294 0.544 1.007 1.821 2.388 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.066 0.098 0.164 0.294 0.544 1.007 1.821 2.388 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.066 0.098 0.164 0.294 0.544 1.007 1.821 2.388 O.D. 0.066 0.098 0.164 0.294 0.544 1.007 1.821 2.388

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.