Human Cystatin B/CSTB ELISA Kit PicoKine®

Human Cystatin B ELISA Kit PicoKine® (96 Tests) . Quantitate Human CSTB in cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Cystatin B (CSTB), also called STFB, is a small protein that is a member of the superfamily of cysteine protease inhibitors. It has been isolated from human spleen and liver and its amino acid sequence has been fully determined. The cystatin B gene is located on 21q22.3. It is widely distributed and is localized mostly intracellularly, but has been found extracellularly. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. Its role is thought to be as a protector against the proteinases leaking from lysosomes. A cystatin B multiprotein complex might have a specific cerebellar function, and that the loss of this function might contribute to the etiopathogenesis of EPM1. Upon differentiation to myotubes, CSTB becomes excluded from the nucleus and lysosomes, suggesting that the subcellular distribution of CSTB is dependent on the differentiation status of the cell.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 108 102 101 101 103 2.91 2.8% Sample 2 522 468 537 457 496 34.13 6.8% Sample 3 1824 1767 1982 1870 1860 78.94 4.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 108 102 101 101 103 2.91 2.8% Sample 1 108 102 101 101 103 2.91 2.8% Sample 2 522 468 537 457 496 34.13 6.8% Sample 2 522 468 537 457 496 34.13 6.8% Sample 3 1824 1767 1982 1870 1860 78.94 4.2% Sample 3 1824 1767 1982 1870 1860 78.94 4.2%

Synonyms

Cystatin-B; CPI-B; Liver thiol proteinase inhibitor; Stefin-B; CSTB; CST6, STFB

Gene Name

CSTB

Gene ID

1476

UniProt

P04080

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: M2-F98

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cell Biology, Protease Inhibitors, Proteolysis/Ubiquitin

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk.

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 108 522 1824 110 532 1878 Standard deviation 7.77 32.88 131.32 9.57 40.96 153.99 CV (%) 7.2% 7.7% 7.2% 8.7% 7.7% 8.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 108 522 1824 110 532 1878 Standard deviation 7.77 32.88 131.32 9.57 40.96 153.99 CV (%) 7.2% 7.7% 7.2% 8.7% 7.7% 8.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 108 522 1824 110 532 1878 Mean (pg/ml) 108 522 1824 110 532 1878 Standard deviation 7.77 32.88 131.32 9.57 40.96 153.99 Standard deviation 7.77 32.88 131.32 9.57 40.96 153.99 CV (%) 7.2% 7.7% 7.2% 8.7% 7.7% 8.2% CV (%) 7.2% 7.7% 7.2% 8.7% 7.7% 8.2%

Sensitivity

<10 pg/mL

Function

This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

11140 MW

Specificity

Natural and recombinant human Cystatin B

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Cystatin-B

Subcellular Location

Cytoplasm . Nucleus .

Sequence Similarities

Belongs to the cystatin family.

Protein Name

Cystatin-B

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.029 0.125 0.213 0.338 0.622 1.054 1.863 2.552 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.029 0.125 0.213 0.338 0.622 1.054 1.863 2.552 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.029 0.125 0.213 0.338 0.622 1.054 1.863 2.552 O.D. 0.029 0.125 0.213 0.338 0.622 1.054 1.863 2.552

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.