Human WISP1/CCN4 ELISA Kit PicoKine®

WISP1 (WNT1-Inducible Signaling Pathway Protein 1), also known as CCN4, is a matricellular protein that in humans is encoded by the WISP1 gene. WISP1 is induced by WNT1 and belongs to the CCN family, which includes connective tissue growth factor, cysteine-rich-61, and nephroblastoma overexpressed. By use of radiation hybrid mapping panels, the WISP1 was mapped to chromosome 8q24.1-q24.3, roughly 4 Mb distal to MYC. It found that 2 distinct systems demonstrated that WISP induction was associated with expression of WNT1. WISP1 genomic DNA was amplified in colon cancer cell lines and in human colon tumors, and its RNA was overexpressed in 84% of tumors examined compared with patient-matched normal mucosa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 98 106 100 90 98 5.72 5.8% Sample 2 231 250 213 236 232 13.23 5.7% Sample 3 917 942 906 865 907 27.78 3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 98 106 100 90 98 5.72 5.8% Sample 1 98 106 100 90 98 5.72 5.8% Sample 2 231 250 213 236 232 13.23 5.7% Sample 2 231 250 213 236 232 13.23 5.7% Sample 3 917 942 906 865 907 27.78 3% Sample 3 917 942 906 865 907 27.78 3%

Expressed in heart, kidney, lung, pancreas, placenta, ovary, small intestine and spleen. Isoform 2 is expressed predominantly in scirrhous gastric carcinoma and, weakly in placenta. Overexpression is associated with several cancers including breast cancer and colon tumors. Isoform 2 is overexpressed in scirrhous gastric carcinoma.

Epigenetics and Nuclear Signaling, Transcription, DNA/RNA, DNA Damage & Repair, DNA Damage Response, Signaling Pathways, Stem Cells, Domain Families, Secreted, Developmental Families

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 98 231 917 94 225 930 Standard deviation 4.48 10.16 46.76 6.95 11.47 47.43 CV (%) 5.8% 4.4% 5.1% 7.4% 5.1% 5.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 98 231 917 94 225 930 Standard deviation 4.48 10.16 46.76 6.95 11.47 47.43 CV (%) 5.8% 4.4% 5.1% 7.4% 5.1% 5.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 98 231 917 94 225 930 Mean (pg/ml) 98 231 917 94 225 930 Standard deviation 4.48 10.16 46.76 6.95 11.47 47.43 Standard deviation 4.48 10.16 46.76 6.95 11.47 47.43 CV (%) 5.8% 4.4% 5.1% 7.4% 5.1% 5.1% CV (%) 5.8% 4.4% 5.1% 7.4% 5.1% 5.1%

Downstream regulator in the Wnt/Frizzled-signaling pathway. Associated with cell survival. Attenuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase. Up-regulates the anti-apoptotic Bcl-X (L) protein. Adheres to skin and melanoma fibroblasts. In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.012 0.035 0.062 0.114 0.252 0.527 1.032 2.041 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.012 0.035 0.062 0.114 0.252 0.527 1.032 2.041 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.012 0.035 0.062 0.114 0.252 0.527 1.032 2.041 O.D. 0.012 0.035 0.062 0.114 0.252 0.527 1.032 2.041

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.