Human CYR61/CCN1 ELISA Kit PicoKine®

Cysteine-rich angiogenic inducer 61 (CYR61) or CCN family member 1 (CCN1), is a matricellular protein that in humans is encoded by the CYR61 gene. This gene is mapped to 1p22.3. CYR61 is capable of regulating a broad range of cellular activities, including cell adhesion, migration, proliferation, differentiation, apoptosis, and senescence through interaction with cell surface integrin receptors and heparan sulfate proteoglycans. During embryonic development, CYR61 is critical for cardiac septal morphogenesis, blood vessel formation in placenta, and vascular integrity. In adulthood CYR61 plays important roles in inflammation and tissue repair, and is associated with diseases related to chronic inflammation, including rheumatoid arthritis, atherosclerosis, diabetes-related nephropathy and retinopathy, and many different forms of cancers.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 61 64 63 58 61 2.29 3.7% Sample 2 248 273 263 255 259 9.31 3.5% Sample 3 814 907 783 869 843 47.99 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 61 64 63 58 61 2.29 3.7% Sample 1 61 64 63 58 61 2.29 3.7% Sample 2 248 273 263 255 259 9.31 3.5% Sample 2 248 273 263 255 259 9.31 3.5% Sample 3 814 907 783 869 843 47.99 5.6% Sample 3 814 907 783 869 843 47.99 5.6%

Protein CYR61; CCN family member 1; Cysteine-rich angiogenic inducer 61; Insulin-like growth factor-binding protein 10; IBP-10; IGF-binding protein 10; IGFBP-10; Protein GIG1; CYR61; CCN1, GIG1, IGFBP10

Angiogenesis, Cancer, Cardiovascular, Extracellular Matrix, Growth Factors, Growth Factors/Hormones, Immunology, Integrins, Mesenchymal Stem Cells, Secreted Molecules, Signal Transduction, Stem Cells

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 61 248 814 59 242 806 Standard deviation 2.86 16.12 55.35 3.3 17.66 64.48 CV (%) 4.7% 6.5% 6.8% 5.6% 7.3% 8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 61 248 814 59 242 806 Standard deviation 2.86 16.12 55.35 3.3 17.66 64.48 CV (%) 4.7% 6.5% 6.8% 5.6% 7.3% 8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 61 248 814 59 242 806 Mean (pg/ml) 61 248 814 59 242 806 Standard deviation 2.86 16.12 55.35 3.3 17.66 64.48 Standard deviation 2.86 16.12 55.35 3.3 17.66 64.48 CV (%) 4.7% 6.5% 6.8% 5.6% 7.3% 8% CV (%) 4.7% 6.5% 6.8% 5.6% 7.3% 8%

Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up- regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.009 0.086 0.156 0.281 0.526 0.96 1.707 2.916 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.009 0.086 0.156 0.281 0.526 0.96 1.707 2.916 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.009 0.086 0.156 0.281 0.526 0.96 1.707 2.916 O.D. 0.009 0.086 0.156 0.281 0.526 0.96 1.707 2.916

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.