Human beta IG-H3/TGFBI ELISA Kit PicoKine®

Transforming growth factor, beta-induced, 68kDa, also known as TGFBI (initially called BIGH3, BIG-H3), is a protein which in humans is encoded by the TGFBI gene. It is mapped to 5q31.1. This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 154 145 147 157 150 4.91 3.2% Sample 2 388 396 437 439 415 23.18 5.5% Sample 3 1132 1229 1284 1106 1187 72.03 6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 154 145 147 157 150 4.91 3.2% Sample 1 154 145 147 157 150 4.91 3.2% Sample 2 388 396 437 439 415 23.18 5.5% Sample 2 388 396 437 439 415 23.18 5.5% Sample 3 1132 1229 1284 1106 1187 72.03 6% Sample 3 1132 1229 1284 1106 1187 72.03 6%

Collagen, Cytoskeleton/ECM, Developmental Biology, ECM Proteins, Extracellular Matrix, Growth Factors/Hormones, Neuroscience, Organogenesis, Sensory System, Signal Transduction, Structures, Visual System

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 154 388 1132 160 387 1210 Standard deviation 6.77 28.32 57.73 7.36 34.41 70.18 CV (%) 4.4% 7.3% 5.8% 4.6% 9.1% 5.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 154 388 1132 160 387 1210 Standard deviation 6.77 28.32 57.73 7.36 34.41 70.18 CV (%) 4.4% 7.3% 5.8% 4.6% 9.1% 5.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 154 388 1132 160 387 1210 Mean (pg/ml) 154 388 1132 160 387 1210 Standard deviation 6.77 28.32 57.73 7.36 34.41 70.18 Standard deviation 6.77 28.32 57.73 7.36 34.41 70.18 CV (%) 4.4% 7.3% 5.8% 4.6% 9.1% 5.8% CV (%) 4.4% 7.3% 5.8% 4.6% 9.1% 5.8%

Binds to type I, II, and IV collagens. This adhesion protein may play an important role in cell-collagen interactions. In cartilage, may be involved in endochondral bone formation.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.031 0.089 0.157 0.305 0.587 1.032 1.818 2.369 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.031 0.089 0.157 0.305 0.587 1.032 1.818 2.369 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.031 0.089 0.157 0.305 0.587 1.032 1.818 2.369 O.D. 0.031 0.089 0.157 0.305 0.587 1.032 1.818 2.369

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.