Human MFGE8/Lactadherin ELISA Kit PicoKine®

Human MFGE8/Lactadherin ELISA Kit PicoKine® (96 Tests) . Quantitate Human MFGE8 in cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

MFGE8 (Milk Fat Globule-Egf Factor 8), also called as Lactadherin or SED1, is a protein which in humans is encoded by the MFGE8 gene. Mfge8 is secreted protein found in vertebrates, including mammals as well as birds. By fluorescence in situ hybridization, the MFGE8 gene was mapped to chromosome 15q25. MFGE8 is a factor that links apoptotic cells to phagocytes. It specifically bound to apoptotic cells by recognizing aminophospholipids such as phosphatidylserine. MFGE8, when engaged by phospholipids, bound to cells via its RGD motif. It bound particularly strongly to cells expressing alpha-V-beta-3 integrin. It showed that Mfge8 was expressed in intestinal lamina propria macrophages in mice. Using a wound-healing assay, they showed that Mfge8 promoted migration of intestinal epithelial cells through a PKC-epsilon (PRKCE) -dependent mechanism.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 100 98 103 96 99 2.58 2.6% Sample 2 660 663 784 706 703 50.04 7.1% Sample 3 1196 1025 1141 1197 1139 70.01 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 100 98 103 96 99 2.58 2.6% Sample 1 100 98 103 96 99 2.58 2.6% Sample 2 660 663 784 706 703 50.04 7.1% Sample 2 660 663 784 706 703 50.04 7.1% Sample 3 1196 1025 1141 1197 1139 70.01 6.1% Sample 3 1196 1025 1141 1197 1139 70.01 6.1%

Synonyms

Lactadherin; Breast epithelial antigen BA46; HMFG; MFGM; Milk fat globule-EGF factor 8; MFG-E8; SED1; Lactadherin short form; Medin; MFGE8

Gene Name

MFGE8

Gene ID

4240

UniProt

Q08431

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L24-C387

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Mammary epithelial cell surfaces and aortic media. Overexpressed in several carcinomas.

Applications

ELISA

Field of Research

Angiogenesis, Apoptosis, Cancer, Cardiovascular, Cell Biology, Cell Death, Metabolism, Metabolism Processes, Pathways and Processes

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk.

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 100 660 1196 101 717 1149 Standard deviation 5.8 36.96 90.89 8.28 41.58 102.26 CV (%) 5.8% 5.6% 7.6% 8.2% 5.8% 8.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 100 660 1196 101 717 1149 Standard deviation 5.8 36.96 90.89 8.28 41.58 102.26 CV (%) 5.8% 5.6% 7.6% 8.2% 5.8% 8.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 100 660 1196 101 717 1149 Mean (pg/ml) 100 660 1196 101 717 1149 Standard deviation 5.8 36.96 90.89 8.28 41.58 102.26 Standard deviation 5.8 36.96 90.89 8.28 41.58 102.26 CV (%) 5.8% 5.6% 7.6% 8.2% 5.8% 8.9% CV (%) 5.8% 5.6% 7.6% 8.2% 5.8% 8.9%

Sensitivity

<10 pg/mL

Function

Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity) . Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

43123 MW

Specificity

Natural and recombinant human MFGE8

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Lactadherin

Subcellular Location

Membrane ; Peripheral membrane protein . Secreted .

Sequence Similarities

Contains 1 EGF-like domain.

Protein Name

Lactadherin

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.006 0.06 0.129 0.256 0.442 0.829 1.384 1.979 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.006 0.06 0.129 0.256 0.442 0.829 1.384 1.979 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.006 0.06 0.129 0.256 0.442 0.829 1.384 1.979 O.D. 0.006 0.06 0.129 0.256 0.442 0.829 1.384 1.979

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.